促进扩散的荧光光漂白回收率测定

Photon Correlation Techniques and Applications Pub Date : 1900-01-01 DOI:10.1364/pcta.1988.pcs159

E. Pike, H. J. Gould, F. Watanabe

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引用次数: 0

摘要

证明了用荧光光漂白恢复技术直接测量蛋白质沿DNA链滑动的可行性。用组蛋白H1获得的结果为理解“促进扩散”机制提供了坚实的基础，通过该机制，位点特异性DNA结合蛋白被认为可以有效地找到其独特的位点。

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Fluorescence Photobleaching Recovery Measurements of Facilitated Diffusion

The feasibility of a direct measurement of sliding of proteins along a DNA chain by the fluorescence photobleaching recovery technique is demonstrated. The results obtained with a protein, histone H1, provides a firm basis for the understanding of the "facilitated diffusion" mechanism by which site-specific DNA binding proteins are thought to find efficiently their unique loci.

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Photon Correlation Techniques and Applications

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