{"title":"硫胺素焦磷酸酶和酸性磷酸酶。它们在大鼠皮肤中的超微结构定位。","authors":"M E Itoiz, B M de Rey, R L Cabrini","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The ultrastructural localization of TPPase and AcPase in rat skin was studied. AcPase appeared localized in the lysosomes of the basal layer and in the keratinosomes of spinous, granulous and corneum layers. A diffuse reaction was also present in the granulous cells cytoplasm. Only the keratinosomes in the superficial layer were TPPase positive, specially those of the intercellular spaces of the proximal horny cells. The Golgi apparatus of all skin cells reacted strongly towards TPP. Membranous structures with TPPase and AcPase activity were evident within horny cells and seem to be infolded portions of the cell membrane which keep their enzymatic activity.</p>","PeriodicalId":8114,"journal":{"name":"Archiv fur dermatologische Forschung","volume":"252 2","pages":"131-7"},"PeriodicalIF":0.0000,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Thiamine pyrophosphatase and acid phosphatase. Their ultrastructural localization in rat skin.\",\"authors\":\"M E Itoiz, B M de Rey, R L Cabrini\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The ultrastructural localization of TPPase and AcPase in rat skin was studied. AcPase appeared localized in the lysosomes of the basal layer and in the keratinosomes of spinous, granulous and corneum layers. A diffuse reaction was also present in the granulous cells cytoplasm. Only the keratinosomes in the superficial layer were TPPase positive, specially those of the intercellular spaces of the proximal horny cells. The Golgi apparatus of all skin cells reacted strongly towards TPP. Membranous structures with TPPase and AcPase activity were evident within horny cells and seem to be infolded portions of the cell membrane which keep their enzymatic activity.</p>\",\"PeriodicalId\":8114,\"journal\":{\"name\":\"Archiv fur dermatologische Forschung\",\"volume\":\"252 2\",\"pages\":\"131-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1975-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archiv fur dermatologische Forschung\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archiv fur dermatologische Forschung","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Thiamine pyrophosphatase and acid phosphatase. Their ultrastructural localization in rat skin.
The ultrastructural localization of TPPase and AcPase in rat skin was studied. AcPase appeared localized in the lysosomes of the basal layer and in the keratinosomes of spinous, granulous and corneum layers. A diffuse reaction was also present in the granulous cells cytoplasm. Only the keratinosomes in the superficial layer were TPPase positive, specially those of the intercellular spaces of the proximal horny cells. The Golgi apparatus of all skin cells reacted strongly towards TPP. Membranous structures with TPPase and AcPase activity were evident within horny cells and seem to be infolded portions of the cell membrane which keep their enzymatic activity.
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