{"title":"讲述的生存问题穷人靠的是液态氮","authors":"P. Smentek, S. Windisch","doi":"10.1016/S0721-9571(82)80027-6","DOIUrl":null,"url":null,"abstract":"<div><p>The storage of 140 strains of yeasts in liquid nitrogen at −196°C has been investigated with respect to composition of the freezing medium and survival of cells. The best results were obtained with a freezing mixture containing dimethylsulfoxide, skimmed milk and Ficoll 400, in which 74% of the strains examined showed survival rates of more than 60%. It is not yet possible to give a generally applicable procedure for optimal survival rates of yeasts under liquid nitrogen, since the various strains react very differently to the freezing conditions.</p></div>","PeriodicalId":101290,"journal":{"name":"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"3 3","pages":"Pages 432-439"},"PeriodicalIF":0.0000,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0721-9571(82)80027-6","citationCount":"2","resultStr":"{\"title\":\"Zur frage des überlebens von hefestämmen unter flüssigem stickstoff\",\"authors\":\"P. Smentek, S. Windisch\",\"doi\":\"10.1016/S0721-9571(82)80027-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The storage of 140 strains of yeasts in liquid nitrogen at −196°C has been investigated with respect to composition of the freezing medium and survival of cells. The best results were obtained with a freezing mixture containing dimethylsulfoxide, skimmed milk and Ficoll 400, in which 74% of the strains examined showed survival rates of more than 60%. It is not yet possible to give a generally applicable procedure for optimal survival rates of yeasts under liquid nitrogen, since the various strains react very differently to the freezing conditions.</p></div>\",\"PeriodicalId\":101290,\"journal\":{\"name\":\"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie\",\"volume\":\"3 3\",\"pages\":\"Pages 432-439\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1982-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0721-9571(82)80027-6\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0721957182800276\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0721957182800276","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Zur frage des überlebens von hefestämmen unter flüssigem stickstoff
The storage of 140 strains of yeasts in liquid nitrogen at −196°C has been investigated with respect to composition of the freezing medium and survival of cells. The best results were obtained with a freezing mixture containing dimethylsulfoxide, skimmed milk and Ficoll 400, in which 74% of the strains examined showed survival rates of more than 60%. It is not yet possible to give a generally applicable procedure for optimal survival rates of yeasts under liquid nitrogen, since the various strains react very differently to the freezing conditions.
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