{"title":"叠氮化钠突变马铃薯耐盐品种筛选","authors":"Hussain, L.A, Al-Miahy,Falah H","doi":"10.54174/utjagr.vo11.n2/32","DOIUrl":null,"url":null,"abstract":"In vitro non- mutated and mutated plants with sodium azide at a concentration of 1 m mol for 30 minutes of three cultivars of Potato (Arnova, Provento and Emma) were screened to salt tolerate. Plants cultured in MS medium supplimented with different levels of sodium chloride (8, 10, 12 and 14 ds m-1) with comparison treatment ( electrical conductivity 6 ds m-1). The cultures were incubated at a temperature of 25 ± 2 and 16 hours day -1. The data were taken on the morphological characteristics of the vegetative growth (plant height, number of shoots and nodes) and the fresh weight of the vegetative group and its content of Na+, K+, Ca++ and amino acid proline. The results showed that the cultivars differed significantly in the morphological characteristics (plant height, number of nodes and the fresh weight of the vegetative growth, Arnova cultivar superior with rates 5.55 cm, 5.72 plant node plant-1 and 236 mg, respectively. Salinity also caused a decrease in the rates of these traits by increasing the salinity levels in addition to its effect in increasing the accumulation of Na+ and decreasing K+, while it had no significant effect on the accumulation of Ca++ and the amino acid proline. Mutation had no significant effect on the accumulation of Ca++ and proline, while an increase K+ in Provento cultivar at all salinity levels compared to 6 ds m-1 and a decrease in the Na+ content at 6, 8 and 14 ds. m-1.","PeriodicalId":101768,"journal":{"name":"University of Thi-Qar Journal of agricultural research","volume":"60 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Screening three cultivars of mutant Potato with sodium azide for salt tolerance\",\"authors\":\"Hussain, L.A, Al-Miahy,Falah H\",\"doi\":\"10.54174/utjagr.vo11.n2/32\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In vitro non- mutated and mutated plants with sodium azide at a concentration of 1 m mol for 30 minutes of three cultivars of Potato (Arnova, Provento and Emma) were screened to salt tolerate. Plants cultured in MS medium supplimented with different levels of sodium chloride (8, 10, 12 and 14 ds m-1) with comparison treatment ( electrical conductivity 6 ds m-1). The cultures were incubated at a temperature of 25 ± 2 and 16 hours day -1. The data were taken on the morphological characteristics of the vegetative growth (plant height, number of shoots and nodes) and the fresh weight of the vegetative group and its content of Na+, K+, Ca++ and amino acid proline. The results showed that the cultivars differed significantly in the morphological characteristics (plant height, number of nodes and the fresh weight of the vegetative growth, Arnova cultivar superior with rates 5.55 cm, 5.72 plant node plant-1 and 236 mg, respectively. Salinity also caused a decrease in the rates of these traits by increasing the salinity levels in addition to its effect in increasing the accumulation of Na+ and decreasing K+, while it had no significant effect on the accumulation of Ca++ and the amino acid proline. Mutation had no significant effect on the accumulation of Ca++ and proline, while an increase K+ in Provento cultivar at all salinity levels compared to 6 ds m-1 and a decrease in the Na+ content at 6, 8 and 14 ds. m-1.\",\"PeriodicalId\":101768,\"journal\":{\"name\":\"University of Thi-Qar Journal of agricultural research\",\"volume\":\"60 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"University of Thi-Qar Journal of agricultural research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.54174/utjagr.vo11.n2/32\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"University of Thi-Qar Journal of agricultural research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54174/utjagr.vo11.n2/32","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
以3个马铃薯品种(Arnova、Provento和Emma)为材料,经1 m mol叠氮化钠处理30 min,筛选了未突变和突变植株的耐盐性。在添加不同水平氯化钠(8、10、12和14 ds - m-1)的MS培养基上培养植株,对照处理(电导率6 ds - m-1)。在25±2和16小时的温度下培养。测定了营养生长的形态特征(株高、芽数、节数)、营养组鲜重及其Na+、K+、Ca++和氨基酸脯氨酸含量。结果表明:各品种在营养生长的形态特征(株高、节数和鲜重)上存在显著差异,其中Arnova品种的株高、节数和鲜重分别为5.55 cm、5.72和236 mg。盐度除了增加Na+的积累、降低K+的积累外,还通过提高盐度水平导致这些性状的速率降低,而对Ca++和氨基酸脯氨酸的积累没有显著影响。突变对Ca++和脯氨酸的积累没有显著影响,而在所有盐度水平下普罗文托品种的K+含量与6 d m-1相比均有所增加,而在6、8和14 d时Na+含量均有所下降。m - 1。
Screening three cultivars of mutant Potato with sodium azide for salt tolerance
In vitro non- mutated and mutated plants with sodium azide at a concentration of 1 m mol for 30 minutes of three cultivars of Potato (Arnova, Provento and Emma) were screened to salt tolerate. Plants cultured in MS medium supplimented with different levels of sodium chloride (8, 10, 12 and 14 ds m-1) with comparison treatment ( electrical conductivity 6 ds m-1). The cultures were incubated at a temperature of 25 ± 2 and 16 hours day -1. The data were taken on the morphological characteristics of the vegetative growth (plant height, number of shoots and nodes) and the fresh weight of the vegetative group and its content of Na+, K+, Ca++ and amino acid proline. The results showed that the cultivars differed significantly in the morphological characteristics (plant height, number of nodes and the fresh weight of the vegetative growth, Arnova cultivar superior with rates 5.55 cm, 5.72 plant node plant-1 and 236 mg, respectively. Salinity also caused a decrease in the rates of these traits by increasing the salinity levels in addition to its effect in increasing the accumulation of Na+ and decreasing K+, while it had no significant effect on the accumulation of Ca++ and the amino acid proline. Mutation had no significant effect on the accumulation of Ca++ and proline, while an increase K+ in Provento cultivar at all salinity levels compared to 6 ds m-1 and a decrease in the Na+ content at 6, 8 and 14 ds. m-1.