[酶动力学参数的可变性。]1. Intermethod振荡)。

Revista de farmacia e bioquimica da Universidade de Sao Paulo Pub Date : 1976-01-01

B C de Almeida Cunha, M Vitolo

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摘要

脲酶活性动力学参数的测定方法有五种，分别是:Henry, Lineweaver-Burk, Hofstee, Woolf和Cornish-Eisenthal。在任何情况下，不同的方法对参数集(Km和Vmáx)产生的值都不相同。

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[Variability of parameters in enzyme kinetics. 1. Intermethod oscillations].

Kinetic parameters of urease activity have been determined by five different methods, namely: Henry, Lineweaver-Burk, Hofstee, Woolf and Cornish-Eisenthal. In no instance different methods yielded the same values for the set of parameters (Km and Vmáx).

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Revista de farmacia e bioquimica da Universidade de Sao Paulo

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