用多“微相”萃取法监测尿氨喷丁胺排泄——一种无需蒸馏从大量生物液中提取和浓缩少量亲脂性药物的快速方法。

W J Serfontein, L S de Villiers
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引用次数: 0

摘要

已经证明,生物液体中低浓度的碱性亲脂药物可以通过一系列提取程序提取和浓缩10(4)-10(6)倍,其中提取溶剂与待提取溶液的比例为1:100(微相提取程序)。通常,通过三个简单的连续提取步骤,从24小时尿液样本中提取和浓缩基本药物(阿托品、氨喷丁胺、莨菪碱、氯喹、乙胺嘧啶)并进行直接GC分析。使用这一程序,证明在以抗腹泻片剂的形式给病人服用氨喷丁胺(300微克)后,可以在24小时的尿液样本中发现可测量数量的游离、不变的药物。该方法具有简单、快速和灵敏度高的优点。所涉及的原理可以推广到具有合适溶解度的酸性药物的分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Monitoring aminopentamide urinary excretion by means of multiple 'microphase' extraction - a rapid method for the extraction and concentration of small amounts of lipophilic drugs from large volumes of biological fluids without distillation.

It has been demonstrated that low concentrations of basic lipophilic drugs in biological fluids may be extracted and concentrated 10(4)-10(6) times by a series of extraction procedures in which the ratio of the extracting solvent to that of the solution to be extracted is of the order of 1:100 (microphase extraction procedure). Typically, basic drugs (atropine, aminopentamide, hyoscine, chloroquine, pyrimethamine) were extracted and concentrated sufficiently for direct GC analysis from 24-hour urine samples by a procedure involving three simple consecutive extraction steps. Using this procedure, it was demonstrated that after administration of aminopentamide (300 micrograms) to patients in the form of anti-diarrhoeal tablets, measurable quantities of the free, unchanged drug can be demonstrated in 24-hour urine samples. The main advantages of the method are simplicity, rapidity and sensitivity due to the low background interference in the GC separations. The principle involved can be extended to the analysis of acidic drugs with suitable solubility properties.

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