柠檬酸缓冲液与抗生素的抗菌作用

O. Gancho, T. Bublii, Oleksij P. Kostyrenko Oleksij P. Kostyrenko, V. I. Fedorchenko
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引用次数: 0

摘要

病原体对药物的抗生素耐药性是一个全球性的基本问题。因此,新的药物组合物是解决这一问题的方法之一。本研究旨在探讨柠檬酸缓冲液与抗生素“阿莫昔拉夫”联合使用对标准微生物参比菌株的抑菌效果。在波尔塔瓦州立医科大学微生物学、病毒学和免疫学系进行的研究中,我们使用了白色念珠菌ATCC10231、大肠杆菌ATCC25922、金黄色葡萄球菌ATCC25923、粪便念珠菌ATCC29213、黄体念珠菌ATCC4698、表皮念珠菌ATCC14990标准培养物。采用基于CLSI的连续稀释定量方法研究了标准微生物菌株对该制剂的敏感性。ISO/TC 212«临床实验室测试和体外诊断测试系统。2021年。”因此,本研究结果清楚地显示了柠檬酸缓冲液和阿莫昔拉夫对所有参考菌株的协同作用。参考菌株金黄色葡萄球菌和表皮葡萄球菌对柠檬酸缓冲液的敏感性最高,是微球菌、肠球菌和真菌的4倍(p < 0.05),是对试验物质最不敏感的大肠杆菌的8倍(p < 0.01)。阿莫昔拉夫对金黄色葡萄球菌ATCC 25923和表皮葡萄球菌ATCC 14990的生长也有抑制作用,对参比菌株大肠杆菌ATCC 25922的最小抑制浓度为葡萄球菌的31.3倍(p < 0.0001)。所提出的柠檬酸缓冲液-阿莫昔拉夫组合可显著提高其成分对所有参考菌株的抑菌效果。因此,大肠杆菌ATCC 25922和溶血弧菌ATCC 4698对该组合的敏感性比单独使用柠檬酸缓冲液或阿莫昔拉夫的敏感性提高了8倍(p < 0.05)。金黄色葡萄球菌和肠球菌对该组合物的敏感性最大:对缓冲液的敏感性可达32倍(p < 0.001),对抗生素的敏感性可达4倍(p < 0.01)。添加阿莫昔拉后,白色念珠菌ATCC10231对柠檬酸缓冲液的敏感性提高了128倍(p < 0.0001)。这是抗生素-柠檬酸盐缓冲剂组合具有协同杀真菌作用的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ANTIMICROBIAL EFFECT OF CITRATE BUFFER WITH ANTIBIOTIC
Antibiotic resistance of pathogens to medications is an essential problem globally. Thus, new medication compositions are one of the ways to solve this problem. This study aimed to investigate the antimicrobial efficacy of the citrate buffer combined with the antibiotic «Amoxiclav» on standard reference strains of microorganisms. We used standard cultures of C. albicans ATCC10231, E. coli ATCC25922, S. aureus ATCC25923, E. faecalis ATCC29213, M. luteus ATCC4698, S. epidermidis ATCC14990 in the study conducted at the Microbiology, Virology and Immunology Department of the Poltava State Medical University. The sensitivity of standard microorganism strains to the composition was studied with a quantitative method of serial dilutions based on CLSI. ISO/TC 212 «Clinical laboratory testing and in vitro diagnostic test systems. 2021». Thus, the results of this study clearly showed a synergistic effect of citrate buffer and amoxiclav on all the reference strains of microorganisms. Reference strains of Staphylococcus aureus and epidermal staphylococcus showed the highest sensitivity to citrate buffer, which was 4 times (p < 0.05) higher than that shown by micrococci, enterococci and fungi, and 8 times (p < 0.01) higher than that of Escherichia coli, which appeared least sensitive to the test substance. Amoxiclav also inhibited the growth of S. aureus ATCC 25923 and S. epidermidis ATCC 14990 and had the minimal effect on the reference strain of E. coli ATCC 25922, the minimal inhibitory concentration of which was 31.3 times (p < 0.0001) higher than that of staphylococci. The proposed citrate buffer-amoxiclav combination significantly increased the antimicrobial efficacy of its components against all the reference strains of microorganisms. Thus, the sensitivity of E. coli ATCC 25922 and M. lysodeikticus ATCC 4698 to the proposed combination increased 8-fold (p < 0.05) compared to their sensitivity to citrate buffer or amoxiclav alone. The sensitivity of Staphylococcus aureus and enterococcі to the composition increased to the maximum: up to 32-fold (p < 0.001) to the buffer and 4‑fold to the antibiotic (p < 0.01). The effect of a significant increase in the sensitivity of C. albicans ATCC10231 strain to the citrate buffer after adding amoxiclav was gone up 128-fold (p < 0.0001). It was the evidence of a synergistic fungicidal action of the antibiotic-citrate buffer combination.
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