Organogenesis optimization in Dendrocalamus asper

Plant tissue culture offers the potential for rapidly increasing selected bamboo clones for conservation and reforestation. Bud break is important for the successful micro propagation in bamboo. An organogenesis optimization protocol is described for Dendrocalamus asper. Nodal explants containing auxiliary buds from 8-10 years old field grown clumps of D. asper were established in Murashige and Skoog’s (MS) medium supplemented with different concentrations of phytohormone 6-benzyelamino purine (BAP) and kinetin. The maximum bud breaking was observed in 6 ppm BAP concentration. Shoot proliferation was found maximum in MS media supplemented with combinations of BAP and kinetin (4.0 ppm BAP, 0.5 ppm kinetin and 4.0 ppm BAP, 1.0 ppm kinetin) after subcultureing the explants.