体外维持犬肥大细胞瘤肥大细胞的超微结构特征。

C M Cobb, H Birkedal-Hansen, F R Denys
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引用次数: 0

摘要

从犬肥大细胞瘤中获得的肥大细胞在细胞培养中维持了11周。体外培养9周后,收集细胞样本进行电镜检查。尽管整体的形态外观足以使它们被识别为肥大细胞,但肿瘤细胞在几个方面与正常组织肥大细胞的描述不同。与正常组织肥大细胞相比,肿瘤细胞表现出微丝的聚积、随机分散的微管和小簇的光滑内质网。肿瘤肥大细胞也呈现出三种不同的颗粒类型:具有无定形和电子致密基质的球形颗粒;不规则形状的颗粒,具有限定的外膜和包含层状和/或卷曲结构的内部基质;含有松散盘绕的无组织膜结构的颗粒,其外观与髓鞘螺旋相似。犬肥大细胞瘤是肥大细胞的极好来源,因为它们可以在不受外来细胞类型污染的情况下大量获得,并且细胞可以在体外维持较长时间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultrastructural characteristics of mast cells from a canine mastocytoma maintained in vitro.

Mast cells obtained from a canine mastocytoma were maintained in cell culture for a period of 11 weeks. Samples of these cells were harvested for electron microscopic examination after 9 weeks in vitro. Although the overall morphologic appearance was sufficient to allow their identification as mast cells, the tumor cells differed in several respects from descriptions of normal tissue mast cells. In contrast to normal tissue mast cells, the tumor cells exhibited peripheral accumulations of microfilaments, randomly dispersed microtubules, and small clusters of smooth endoplasmic reticulum. The tumor mast cells also presented three different granule types: spherical granules with an amorphous and electron dense matrix; irregularly shaped granules possessing a limiting external membrane and an internal matrix containing laminated and/or coiled structures; and granules containing loosely coiled, unorganized membrane structures similar in appearance to myelin whorls. The canine mastocytoma is an excellent source of mast cells as they can be obtained in large numbers without contamination by extraneous cell types and the cells can be maintained in vitro for extended periods of time.

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