豚鼠血浆胆固醇酯化活性。

R Ostwald, M Green, M Meng
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引用次数: 6

摘要

血浆胆固醇酯化活性在豚鼠中进行了测量,这些豚鼠分别饲喂对照饮食和添加1%胆固醇的相同饮食。在喂食胆固醇的豚鼠和对照豚鼠中发现的酯化程度相似,但略低于大鼠。初始的酯化速率也与在大鼠和人类中发现的速率相同,并且如果使用自体血浆作为底物,则不受饮食胆固醇的影响。然而,当作用于对照或胆固醇喂养的底物时,来自胆固醇喂养的豚鼠的LCAT活性显著高于对照血浆。这表明,膳食胆固醇增加了LCAT的数量(或活性),但底物不合适,或者必要的辅助因子存在于有限的量。豚鼠血浆的热处理似乎在不同程度上改变了基底的可用性。讨论了这些发现与豚鼠LCAT的底物特异性和辅因子需求相关的意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Plasma cholesterol esterifying activity in guinea pigs.

Plasma cholesterol esterifying activity has been measured in guinea pigs fed either a control diet or the same diet supplemented with 1% cholesterol. The extent of esterification was found to be similar in the cholesterol-fed and control guinea pigs and somewhat lower than in rats. The initial rate of esterification was also of the same magnitude as that found in rats and humans, and unaffected by dietary cholesterol if autologous plasma was used as substrate. However, LCAT activity from cholesterol-fed guinea pigs was significantly higher than that of control plasma when acting on either control or cholesterol-fed substrate. This suggests that dietary cholesterol increases the amount (or activity) of LCAT but that the substrate is unsuitable or that a necessary cofactor is present in limiting amounts. Heat treatment of guinea pig plasma seems to alter substrate availability to varying degrees. The implications of these findings in relation to substrate specificity and cofactor requirements of guinea pig LCAT are discussed.

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