{"title":"DNA在染色质和核小体亚基中的构象变化","authors":"Robert C. Krueger","doi":"10.1016/0161-5890(78)90110-4","DOIUrl":null,"url":null,"abstract":"<div><p>Conformation of the DNA in a salt-soluble chromatin and in the nucleosome sub-unit was studied in a variety of ionic environments. Precipitation with Mg<sup>2+</sup> was used as one kind of a probe to indicate the arrangement of the proteins in the complex. Parallel circular dichroic measurements were also made. Three types of DNA conformation have been described: (1) an extended configuration. (2) an intermediate type as observed in the high mol. wt, salt-soluble chromatin and (3) the type seen in the nucleosome sub-unit. Both the chromatin and the nueleosome are pictured as flexible complexes iheir structure being highly dependent on the ionic environment.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 10","pages":"Pages 787-790"},"PeriodicalIF":0.0000,"publicationDate":"1978-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90110-4","citationCount":"1","resultStr":"{\"title\":\"Conformational changes of DNA in chromatin and the nucleosome sub-unit\",\"authors\":\"Robert C. Krueger\",\"doi\":\"10.1016/0161-5890(78)90110-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Conformation of the DNA in a salt-soluble chromatin and in the nucleosome sub-unit was studied in a variety of ionic environments. Precipitation with Mg<sup>2+</sup> was used as one kind of a probe to indicate the arrangement of the proteins in the complex. Parallel circular dichroic measurements were also made. Three types of DNA conformation have been described: (1) an extended configuration. (2) an intermediate type as observed in the high mol. wt, salt-soluble chromatin and (3) the type seen in the nucleosome sub-unit. Both the chromatin and the nueleosome are pictured as flexible complexes iheir structure being highly dependent on the ionic environment.</p></div>\",\"PeriodicalId\":13265,\"journal\":{\"name\":\"Immunochemistry\",\"volume\":\"15 10\",\"pages\":\"Pages 787-790\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0161-5890(78)90110-4\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0161589078901104\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078901104","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Conformational changes of DNA in chromatin and the nucleosome sub-unit
Conformation of the DNA in a salt-soluble chromatin and in the nucleosome sub-unit was studied in a variety of ionic environments. Precipitation with Mg2+ was used as one kind of a probe to indicate the arrangement of the proteins in the complex. Parallel circular dichroic measurements were also made. Three types of DNA conformation have been described: (1) an extended configuration. (2) an intermediate type as observed in the high mol. wt, salt-soluble chromatin and (3) the type seen in the nucleosome sub-unit. Both the chromatin and the nueleosome are pictured as flexible complexes iheir structure being highly dependent on the ionic environment.
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