Luigi G. Marzilli, Brian F. Hanson, Leilani Kapili, Seth D. Rose, Michael Beer
{"title":"锇标记的多核苷酸:四氧化锇与聚1,n6 -乙烯腺苷酸的反应","authors":"Luigi G. Marzilli, Brian F. Hanson, Leilani Kapili, Seth D. Rose, Michael Beer","doi":"10.1016/0006-3061(78)80006-4","DOIUrl":null,"url":null,"abstract":"<div><p>Osmium tetraoxide, in the presence of ligands such as pyridine and bipyridine, adds across the etheno bridge of 1<em>N</em><sup>6</sup>-etheno-9-methyladenine and poly-1 <em>N</em><sup>6</sup>-ethenoadenylic acid. The Os:P ratio in the labeled polynucleotide was 1 when bipyridine was used as the stabilizing ligands. A similar study with polycytidylic acid, which had been partially modified with chloroacetaldehyde so that some bases were converted to 3 <em>N</em><sup>4</sup>-ethenocytosine, gave an Os:P ration of 1.3. Calf-thymus DNA, in which the adenine and cytosine bases were modified by chloroacetaldehyde, gave an Os:P ratio of 1 after 24 h. These results suggest that 3 <em>N</em><sup>4</sup>-ethenocytosine will add two Os labels.</p></div>","PeriodicalId":9177,"journal":{"name":"Bioinorganic chemistry","volume":"8 6","pages":"Pages 531-534"},"PeriodicalIF":0.0000,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3061(78)80006-4","citationCount":"3","resultStr":"{\"title\":\"Osmium-labeled Polynucleotides: Reaction of Osmium Tetraoxide, with Poly-1,N6-Ethenoadenylic Acid\",\"authors\":\"Luigi G. Marzilli, Brian F. Hanson, Leilani Kapili, Seth D. Rose, Michael Beer\",\"doi\":\"10.1016/0006-3061(78)80006-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Osmium tetraoxide, in the presence of ligands such as pyridine and bipyridine, adds across the etheno bridge of 1<em>N</em><sup>6</sup>-etheno-9-methyladenine and poly-1 <em>N</em><sup>6</sup>-ethenoadenylic acid. The Os:P ratio in the labeled polynucleotide was 1 when bipyridine was used as the stabilizing ligands. A similar study with polycytidylic acid, which had been partially modified with chloroacetaldehyde so that some bases were converted to 3 <em>N</em><sup>4</sup>-ethenocytosine, gave an Os:P ration of 1.3. Calf-thymus DNA, in which the adenine and cytosine bases were modified by chloroacetaldehyde, gave an Os:P ratio of 1 after 24 h. These results suggest that 3 <em>N</em><sup>4</sup>-ethenocytosine will add two Os labels.</p></div>\",\"PeriodicalId\":9177,\"journal\":{\"name\":\"Bioinorganic chemistry\",\"volume\":\"8 6\",\"pages\":\"Pages 531-534\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0006-3061(78)80006-4\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioinorganic chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0006306178800064\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioinorganic chemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0006306178800064","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Osmium-labeled Polynucleotides: Reaction of Osmium Tetraoxide, with Poly-1,N6-Ethenoadenylic Acid
Osmium tetraoxide, in the presence of ligands such as pyridine and bipyridine, adds across the etheno bridge of 1N6-etheno-9-methyladenine and poly-1 N6-ethenoadenylic acid. The Os:P ratio in the labeled polynucleotide was 1 when bipyridine was used as the stabilizing ligands. A similar study with polycytidylic acid, which had been partially modified with chloroacetaldehyde so that some bases were converted to 3 N4-ethenocytosine, gave an Os:P ration of 1.3. Calf-thymus DNA, in which the adenine and cytosine bases were modified by chloroacetaldehyde, gave an Os:P ratio of 1 after 24 h. These results suggest that 3 N4-ethenocytosine will add two Os labels.
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