DPPH(1,1二苯基-2-苦基肼)的抗氧化活性研究

S. Husein, E. Safitri, Yovita Endah Lestari
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引用次数: 0

摘要

在东南亚,如印度尼西亚、泰国、菲律宾和马来西亚,人们普遍食用白太白龙或白太白。本文利用DPPH(1,1二苯基-2-苦基肼)分析了白菖蒲的抗氧化活性。从植物化学分析、DPPH降解和动力学降解等方面证明了其抗氧化活性。采用不同试剂滴滴法对枇杷龙提取物进行植物化学分析。DPPH与提取液分别以8 ppm、12 ppm、16 ppm、20 ppm、24 ppm的浓度混合进行DPPH。采用紫外-可见分光光度法进行降解分析,采用Langmuir Hinshelwood (L-H)法计算降解动力学。结果表明,龙柏具有一定的抗氧化活性。所述,最高降解发生在24ppm,其中其降解约10ppm的DPPH,或约96.14%,并且最低降解发生在8ppm,其中其降解约2ppm的DPPH,或约0.98%。Langmuir Hinselwood计算结果表明,DPPH的动力学降解随着petai selong浓度的增加而增加。结果表明,贝塞龙浓度越高,抗氧化效果越好。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ANTIOXIDANT ACTIVITY OF PETAI SELONG (Leucaena leucocephala) USING DPPH (1,1 diphenyl-2-picrylhydrazl)
Petai selong or petai china (leucaena leucocephala) is populary consumed in southeast asia, such as indonesia, thailand, the philippines and malaysia. This paper aims to anlayze the antioxidant activity of  petai selong (leucaena leucocephala) using DPPH(1,1 diphenyl-2-picrylhydrazl). The antioxidant activity is shown from the phytochemical analysis, degradation of DPPH, and kinetics degradation.  Phytochemical analysis was carried out by dripping petai selong extract with various reagent.DPPH was conducted by mixing the DPPH and extract petai selong with 8 ppm, 12 ppm, 16 ppm, 20 ppm, and 24 ppm. The degradation analysis was conduted using spectrofotometer UV-Vis, and the kinetics degradation were calculated using Langmuir Hinshelwood (L-H). The results show that the petai selong has an antioxidant acitivty. It is stated that the highest degradation occurs at 24 ppm where it degrades about 10 ppm of DPPH, or approximatly about96.14 percent, and the lowest degradation occurs at 8 ppm where it degrades about 2 ppm of DPPH, or approximatly about 0.98 percent. The Langmuir Hinselwood calculations show that the kinetics degradations of DPPH increase with petai selong concentration. It is stated that the higher of petai selong concentrations, the higher of antioxidant effect. 
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