B. Filipović, B. Šošić-Jurjević, M. Manojlović-Stojanoski, N. Nestorović, V. Milošević, M. Sekulić
{"title":"卵巢切除对成年大鼠甲状腺c细胞的影响","authors":"B. Filipović, B. Šošić-Jurjević, M. Manojlović-Stojanoski, N. Nestorović, V. Milošević, M. Sekulić","doi":"10.2298/JMH0204345F","DOIUrl":null,"url":null,"abstract":"Summary: The structure and function of C cells of adult female rats after ovariectomy (Ovx) were investigated. Intact control and Ovx rats were i.p. treated with sterile olive oil for 4 weeks. Peroxidase-antiperoxidase (PAP) immunohistochemical procedure was applied to localize calcitonin (CT) in thyroid C cells while its serum content was determined by RIA method. Morphometric analyses of the C cells volume, that of their nuclei and relative volume density included stereological method with the multipurpose test system M42. Also, the average number of C cells number per mm2 was calculated. Ovx led to a significant increase in body weight (21%; p<0.005). At the same time the C cells of Ovx rats had a significantly decreased cell volume (13%; p<0.005) and their number per mm2 was increased by 59% (p<0.001) in comparison with the controls. Ovx resulted in reduction of serum CT level by 45% comparing to the corresponding controls. Based on these data it can be concluded that Ovx inhibits both the structure and function of the C cells.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"209 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"THE EFFECT OF OVARIECTOMY ON THYROID C CELLS OF ADULT RATS\",\"authors\":\"B. Filipović, B. Šošić-Jurjević, M. Manojlović-Stojanoski, N. Nestorović, V. Milošević, M. Sekulić\",\"doi\":\"10.2298/JMH0204345F\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Summary: The structure and function of C cells of adult female rats after ovariectomy (Ovx) were investigated. Intact control and Ovx rats were i.p. treated with sterile olive oil for 4 weeks. Peroxidase-antiperoxidase (PAP) immunohistochemical procedure was applied to localize calcitonin (CT) in thyroid C cells while its serum content was determined by RIA method. Morphometric analyses of the C cells volume, that of their nuclei and relative volume density included stereological method with the multipurpose test system M42. Also, the average number of C cells number per mm2 was calculated. Ovx led to a significant increase in body weight (21%; p<0.005). At the same time the C cells of Ovx rats had a significantly decreased cell volume (13%; p<0.005) and their number per mm2 was increased by 59% (p<0.001) in comparison with the controls. Ovx resulted in reduction of serum CT level by 45% comparing to the corresponding controls. Based on these data it can be concluded that Ovx inhibits both the structure and function of the C cells.\",\"PeriodicalId\":287983,\"journal\":{\"name\":\"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry\",\"volume\":\"209 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2298/JMH0204345F\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/JMH0204345F","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
THE EFFECT OF OVARIECTOMY ON THYROID C CELLS OF ADULT RATS
Summary: The structure and function of C cells of adult female rats after ovariectomy (Ovx) were investigated. Intact control and Ovx rats were i.p. treated with sterile olive oil for 4 weeks. Peroxidase-antiperoxidase (PAP) immunohistochemical procedure was applied to localize calcitonin (CT) in thyroid C cells while its serum content was determined by RIA method. Morphometric analyses of the C cells volume, that of their nuclei and relative volume density included stereological method with the multipurpose test system M42. Also, the average number of C cells number per mm2 was calculated. Ovx led to a significant increase in body weight (21%; p<0.005). At the same time the C cells of Ovx rats had a significantly decreased cell volume (13%; p<0.005) and their number per mm2 was increased by 59% (p<0.001) in comparison with the controls. Ovx resulted in reduction of serum CT level by 45% comparing to the corresponding controls. Based on these data it can be concluded that Ovx inhibits both the structure and function of the C cells.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
