G. Emi-Reynolds, S. Zaki, G. Banini, S. Dogbe, Ma Ofori-Appiah, I. Ennison, A. Fianko, Jy Faanu, C. Schandorf
{"title":"蟹壳中甲壳素和壳聚糖的辐射加工及表征","authors":"G. Emi-Reynolds, S. Zaki, G. Banini, S. Dogbe, Ma Ofori-Appiah, I. Ennison, A. Fianko, Jy Faanu, C. Schandorf","doi":"10.4314/JGSA.V9I2.18009","DOIUrl":null,"url":null,"abstract":"The extraction and characterization of Chitin and Chitosan from crab shells (Callinectes sp.) obtained locally in Ghana is presented. The shells were finely milled and soaked in 10 % dilute hydrochloric acid (HCl) for 48 hr followed by de-proteinization using 2M sodium hydroxide (NaOH) solution for 24 hr to obtain Chitin. The Chitin was refluxed at 100 oC in 50 % NaOH for 7 hr to yield Chitosan. The Chitin and Chitosan were characterized by determining the de-acetylation, viscosity and average molecular weights. The degree of de-acetylation was determined to be 89.7 %. The viscosity of Chitosan in dilute acetic acid was measured and the average molecular weight estimated. The average molecular weight of dry gamma irradiated (up to 100kGy) Chitosan samples decreased with increasing dose. The results have been discussed in terms of radiation induced degradation of\nsolids. Journal of the Ghana Science Association Vol. 9 (2) 2007: pp. 18-24","PeriodicalId":301438,"journal":{"name":"Journal of the Ghana Science Association","volume":"26 4 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2009-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Radiation Processing And Characterization Of Chitin And Chitosan Extracted From Crab Shells\",\"authors\":\"G. Emi-Reynolds, S. Zaki, G. Banini, S. Dogbe, Ma Ofori-Appiah, I. Ennison, A. Fianko, Jy Faanu, C. Schandorf\",\"doi\":\"10.4314/JGSA.V9I2.18009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The extraction and characterization of Chitin and Chitosan from crab shells (Callinectes sp.) obtained locally in Ghana is presented. The shells were finely milled and soaked in 10 % dilute hydrochloric acid (HCl) for 48 hr followed by de-proteinization using 2M sodium hydroxide (NaOH) solution for 24 hr to obtain Chitin. The Chitin was refluxed at 100 oC in 50 % NaOH for 7 hr to yield Chitosan. The Chitin and Chitosan were characterized by determining the de-acetylation, viscosity and average molecular weights. The degree of de-acetylation was determined to be 89.7 %. The viscosity of Chitosan in dilute acetic acid was measured and the average molecular weight estimated. The average molecular weight of dry gamma irradiated (up to 100kGy) Chitosan samples decreased with increasing dose. The results have been discussed in terms of radiation induced degradation of\\nsolids. Journal of the Ghana Science Association Vol. 9 (2) 2007: pp. 18-24\",\"PeriodicalId\":301438,\"journal\":{\"name\":\"Journal of the Ghana Science Association\",\"volume\":\"26 4 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-01-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Ghana Science Association\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4314/JGSA.V9I2.18009\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Ghana Science Association","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/JGSA.V9I2.18009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
摘要
介绍了从加纳当地产的蟹壳(Callinectes sp.)中提取几丁质和壳聚糖的方法。将壳磨细，在10%稀盐酸(HCl)中浸泡48小时，再用2M氢氧化钠(NaOH)溶液脱蛋白24小时，得到几丁质。将甲壳素在100℃、50% NaOH条件下回流7小时，制得壳聚糖。通过测定甲壳素和壳聚糖的去乙酰化程度、粘度和平均分子量对其进行了表征。去乙酰化度为89.7%。测定了壳聚糖在稀乙酸中的粘度，测定了壳聚糖的平均分子量。干γ辐照后(辐照强度为100kGy)壳聚糖样品的平均分子量随辐照剂量的增加而降低。从辐射诱导固体降解的角度对结果进行了讨论。加纳科学协会杂志Vol. 9 (2) 2007: pp. 18-24
Radiation Processing And Characterization Of Chitin And Chitosan Extracted From Crab Shells
The extraction and characterization of Chitin and Chitosan from crab shells (Callinectes sp.) obtained locally in Ghana is presented. The shells were finely milled and soaked in 10 % dilute hydrochloric acid (HCl) for 48 hr followed by de-proteinization using 2M sodium hydroxide (NaOH) solution for 24 hr to obtain Chitin. The Chitin was refluxed at 100 oC in 50 % NaOH for 7 hr to yield Chitosan. The Chitin and Chitosan were characterized by determining the de-acetylation, viscosity and average molecular weights. The degree of de-acetylation was determined to be 89.7 %. The viscosity of Chitosan in dilute acetic acid was measured and the average molecular weight estimated. The average molecular weight of dry gamma irradiated (up to 100kGy) Chitosan samples decreased with increasing dose. The results have been discussed in terms of radiation induced degradation of
solids. Journal of the Ghana Science Association Vol. 9 (2) 2007: pp. 18-24
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
