Jorge Díaz Heredia , Susana Alonso Güemes , Adrián Cuéllar Ayestarán , Miguel Angel Ruiz Iban
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At the same time 50<!--> <!-->g of abdominal fat was removed and 2<!--> <!-->cm<sup>3</sup> of SVF was purified (in which it was found to contain approximately 1-5<!--> <!-->×<!--> <!-->10<sup>6</sup> <!-->mesenchymal stem cells). This was injected into one of the knees of each pig 4<!--> <!-->h after the surgery (SVF group), using the other knee as the control group. The animals were sacrificed after 15 days. The following histological parameters with hematoxylin-eosin staining were evaluated: degree of healing, neo-vascularization of tissue and the proliferation of fibro-chondrocytes around the lesion and the presence of new chondrocytes.</p></div><div><h3>Results</h3><p>The healing rate (unhealed / partial healing / healed) for the SVF group was 1/1/2, and was 2-2-0 for control group (non-significant differences). The degree of neo-vascularization was higher in the SVF group (3.25 vs 2). The degree of fibro-chondrocytes proliferation was greater in the SVF group (2.5 vs 1.75). The formation of isogenic chondrocytes was observed in 2 menisci of the SVF group and none in the control group.</p></div><div><h3>Conclusions</h3><p>The postoperative intra-articular injection of SVF might increase the neovascularization, the proliferation of fibro-chondrocytes and the repopulation of chondrocytes 15 days after the repair of meniscal injury in pigs.</p></div><div><h3>Evidence level</h3><p>Experimental study.</p></div><div><h3>Clinical relevance</h3><p>A system for the use of stem cells is presented that might be easier to implement in humans that cell cultures.</p></div>","PeriodicalId":101107,"journal":{"name":"Revista Espa?ola de Artroscopia y Cirugía Articular","volume":"21 2","pages":"Pages 95-100"},"PeriodicalIF":0.0000,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.reaca.2014.12.001","citationCount":"1","resultStr":"{\"title\":\"Efecto de la adición de fracción vasculoestromal de grasa a la sutura de lesiones meniscales crónicas en zona avascular del menisco de cerdo\",\"authors\":\"Jorge Díaz Heredia , Susana Alonso Güemes , Adrián Cuéllar Ayestarán , Miguel Angel Ruiz Iban\",\"doi\":\"10.1016/j.reaca.2014.12.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>The addition of mesenchymal stem cells may increase the chance of healing of meniscal lesions in the avascular zone. 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引用次数: 1
摘要
目的间充质干细胞的加入可增加半月板无血管区病变的愈合机会。本研究的目的是探讨术后应用脂肪组织基质血管组分(SVF)对缝合后半月板血管化和细胞增殖是否有影响。方法4头猪(8膝)双膝内侧半月板无血管区纵损伤10 mm。两个月后，双膝伤口用简单的缝线修复。同时去除腹部脂肪50 g，纯化SVF 2 cm3(其中发现含有约1-5 × 106个间充质干细胞)。术后4小时，将该药注射到每只猪的一个膝盖(SVF组)，另一个膝盖作为对照组。这些动物在15天后被处死。用苏木精-伊红染色评估以下组织学参数:愈合程度、组织新生血管化、病变周围纤维软骨细胞增殖和新软骨细胞的存在。结果SVF组愈合率(未愈合/部分愈合/愈合)为1/1/2，对照组为2-2-0，差异无统计学意义。SVF组新生血管形成程度更高(3.25 vs 2)，纤维软骨细胞增殖程度更高(2.5 vs 1.75)。SVF组2例半月板出现等基因软骨细胞形成，对照组无。结论关节内注射SVF可促进半月板损伤修复后15 d的新生血管形成、纤维软骨细胞增殖和软骨细胞再生。证据水平实验研究。临床相关性提出了一种使用干细胞的系统，该系统可能比细胞培养更容易在人类中实施。
Efecto de la adición de fracción vasculoestromal de grasa a la sutura de lesiones meniscales crónicas en zona avascular del menisco de cerdo
Objective
The addition of mesenchymal stem cells may increase the chance of healing of meniscal lesions in the avascular zone. The objective of this study is to find out if the postoperative application of the stromal vascular fraction (SVF) from adipose tissue can have an effect on vascularization and cell proliferation in the sutured meniscus.
Methods
Four pigs (8 knees) underwent a longitudinal injury of 10 mm in the avascular area of the medial meniscus in both knees. Two months later the injury was repaired on both knees with a simple suture. At the same time 50 g of abdominal fat was removed and 2 cm3 of SVF was purified (in which it was found to contain approximately 1-5 × 106 mesenchymal stem cells). This was injected into one of the knees of each pig 4 h after the surgery (SVF group), using the other knee as the control group. The animals were sacrificed after 15 days. The following histological parameters with hematoxylin-eosin staining were evaluated: degree of healing, neo-vascularization of tissue and the proliferation of fibro-chondrocytes around the lesion and the presence of new chondrocytes.
Results
The healing rate (unhealed / partial healing / healed) for the SVF group was 1/1/2, and was 2-2-0 for control group (non-significant differences). The degree of neo-vascularization was higher in the SVF group (3.25 vs 2). The degree of fibro-chondrocytes proliferation was greater in the SVF group (2.5 vs 1.75). The formation of isogenic chondrocytes was observed in 2 menisci of the SVF group and none in the control group.
Conclusions
The postoperative intra-articular injection of SVF might increase the neovascularization, the proliferation of fibro-chondrocytes and the repopulation of chondrocytes 15 days after the repair of meniscal injury in pigs.
Evidence level
Experimental study.
Clinical relevance
A system for the use of stem cells is presented that might be easier to implement in humans that cell cultures.
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