Characterization of β-TCP, β-TCMP and BCMP Produced by Hydrolysis

Calcium Deficient Apatites (CDA’s) were prepared by hydrolysis method. The CDA’s were filtered, washed and calcined at 950⁰C for 11h. X-ray diffraction demonstrated that β-TCP was obtained after calcining Mg-free CDA and β-TCMP or BCMP were obtained after calcining Mg-substituted CDAs, depending on the Mg/Ca molar ratio of the CDA. Physicochemical characterization was also performed by FT-IR spectroscopy and inductive coupled plasma. Phenolphthalein test was performed, in order to investigate the presence of calcium oxide according to the French standard NF S 94-066. SEM images assessed the morphology of the compounds. Cell viability assay (MTT), calcium nodule formation and the expression of alkaline phosphatase (ALP), osteocalcin, TGF-β1 and collagen were performed in MC3T3-E1 cell line. β-TCP, β-TCMP and BCMP obtained from hydrolysis method weren’t toxic and promoted cell proliferation, showing potential value in bone tissue engineering.