抗肾质膜抗体对(Na+K+)- atp酶和阳离子转运的抑制作用。

H Ebel, E Aulbert, R Averdunk
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引用次数: 0

摘要

在家兔中培养了一种抗NaI大鼠肾质膜的抗体。该抗体抑制基底膜和大鼠红细胞内的(Na+K+)- atp酶。K+刺激的pNPase也被抑制。Mg-ATPase和基础pNPase活性未受影响。Na+、K+和Mg-ATP的动力学实验表明,该抗体对(Na+K+)- atp酶具有非竞争性抑制作用。Hill常数不变。在肾上皮细胞培养和完整的大鼠红细胞中,该抗体可抑制瓦巴因敏感的22na外排和42K内流。结果表明,向外Na+K+- atp酶具有免疫原性。当抗原被充分分解以打开隐藏的抗原位点时,抗体可以被提高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inhibition of (Na+K+)-ATPase and cation transport by an antibody against renal plasma membranes.

An antibody was raised in rabbits against NaI extracted plasma membranes of rat kidney. The antibody inhibited (Na+K+)-ATPase of basolateral membranes and of rat erythrocyte ghosts. Also K+-stimulated pNPase was inhibited. No effect on Mg-ATPase and basal pNPase activity was registered. Kinetic experiments with Na+,K+ and Mg-ATP showed a noncompetitive inhibition of (Na+K+)-ATPase by the antibody. Hill constants were unchanged. In renal epithelial cell cultures as well as in intact rat erythrocytes ouabain sensitive 22Na-efflux and 42K influx was inhibited by the antibody. It is concluded that outward facing Na+K+-ATPase is immunogenic. The antibody can be raised when the antigen is adequately disintegrated to open otherwise hidden antigenic sites.

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