与肽聚糖化学相关的合成免疫原的免疫化学研究。

P H Seidl, K H Schleifer
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引用次数: 0

摘要

兔分别用合成免疫原HSA-(Gly-L-Ala-L-Ala-D-Ala-D-Ala)39和HSA-(gly - γ - d - glu - l - ala - d - ala - d - ala)40免疫。抗HSA-(Gly-L-Ala-L-Ala-D-Ala-D-Ala - d -ala)39的抗体与同源抗原、HSA-(gly - γ - d - glu - l- ala - d -ala - d -ala)40和含有c端d -丙烯酰- d -丙氨酸肽亚基的可溶性肽聚糖表现出强烈的沉淀反应。白蛋白-肽偶联物也与兔抗血清发生交叉反应,抗血清含有针对肽聚糖肽段的抗体。通过Ouchterlony凝胶扩散研究和适当的定量沉淀反应抑制试验,证明了a型链球菌和HSA-(Gly-L-Ala-L-Ala-D-Ala-D-Ala)39的肽聚糖决定基团相同。用HSA-(gly - γ - d - glu - l - ala - d - ala - d - ala - d - ala)40免疫家兔产生的抗血清除了对HSA-(Gly-L-Ala-L-Ala-D-Ala-D-Ala)39具有特异性外,还显示出额外的特异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunochemical studies with synthetic immunogens chemically related to peptidoglycan.

Rabbits were immunized with synthetic immunogens HSA-(Gly-L-Ala-L-Ala-D-Ala-D-Ala)39 and HSA-(Gly-gamma-D-Glu-L-Ala-D-Ala-D-Ala)40, respectively. Antibodies against HSA-(Gly-L-Ala-L-Ala-D-Ala-D-Ala)39 showed a strong precipitin reaction with the homologous antigen, with HSA-(Gly-gamma-D-Glu-L-Ala-D-Ala-D-Ala)40 and with solubilized peptidoglycan containing peptide subunits with C-terminal D-alanyl-D-alanine. The albumin-peptide conjugates also cross-reacted with rabbit antisera to Streptococcus A-variant, which contain antibodies directed against the peptide moiety of peptidoglycan. The proof for identical determinant groups of peptidoglycan of Streptococcus A-variant and HSA-(Gly-L-Ala-L-Ala-D-Ala-D-Ala)39 was furnished by Ouchterlony gel diffusion studies and by the appropriate inhibition tests of the quantitative precipitin reaction. Immunization of rabbits with HSA-(Gly-gamma-D-Glu-L-Ala-D-Ala-D-Ala)40 yielded antisera which, besides the specificity of antisera to HSA-(Gly-L-Ala-L-Ala-D-Ala-D-Ala)39, showed an additional specificity.

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