{"title":"亲和层析法分离抗触珠蛋白抗体","authors":"Jerzy Osada, Wanda Dobryszycka","doi":"10.1016/0161-5890(78)90013-5","DOIUrl":null,"url":null,"abstract":"<div><p>Antibodies directed against human haptoglobin type 1-1 and to porcine haptoglobin were isolated from rabbit antisera by means of affinity chromatography on Sepharose 4B. The obtained antibodies were characterized by the quantitative precipitin reaction, double immunodiffusion, immunoelectrophoresis and ultracentrifugation.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 8","pages":"Pages 591-593"},"PeriodicalIF":0.0000,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90013-5","citationCount":"2","resultStr":"{\"title\":\"Isolation of anti-haptoglobin antibodies by affinity chromatography\",\"authors\":\"Jerzy Osada, Wanda Dobryszycka\",\"doi\":\"10.1016/0161-5890(78)90013-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Antibodies directed against human haptoglobin type 1-1 and to porcine haptoglobin were isolated from rabbit antisera by means of affinity chromatography on Sepharose 4B. The obtained antibodies were characterized by the quantitative precipitin reaction, double immunodiffusion, immunoelectrophoresis and ultracentrifugation.</p></div>\",\"PeriodicalId\":13265,\"journal\":{\"name\":\"Immunochemistry\",\"volume\":\"15 8\",\"pages\":\"Pages 591-593\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0161-5890(78)90013-5\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0161589078900135\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078900135","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation of anti-haptoglobin antibodies by affinity chromatography
Antibodies directed against human haptoglobin type 1-1 and to porcine haptoglobin were isolated from rabbit antisera by means of affinity chromatography on Sepharose 4B. The obtained antibodies were characterized by the quantitative precipitin reaction, double immunodiffusion, immunoelectrophoresis and ultracentrifugation.