四氧嘧啶型糖尿病大鼠脑和肝脏谷氨酰胺合成酶和γ -谷氨酰转移酶活性(IV)。

Voprosy biokhimii mozga Pub Date : 1976-01-01
V B Egian, G A Turshian, G E Akopian, S S Safrazian, G Kh Buniatian
{"title":"四氧嘧啶型糖尿病大鼠脑和肝脏谷氨酰胺合成酶和γ -谷氨酰转移酶活性(IV)。","authors":"V B Egian,&nbsp;G A Turshian,&nbsp;G E Akopian,&nbsp;S S Safrazian,&nbsp;G Kh Buniatian","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Glutamine synthetase and gamma-glutamyltransferase activities of brain and liver homogenates of rats suffering from alloxan diabetes were determined in the soluble fraction (fraction 1) and in that obtained after treatment with 0.2 percent deoxycholate (fraction 2). The results obtained indicate that the activities of these enzymes in homogenates of brain and liver of diabetic animals does not differ from that of normal animals. gamma-Glutamyltransferase activity of brain is significantly reduced (about 5 fold) in the soluble fraction while glutamine synthetase activity is not much changed. The activities of glutamine-synthetase and gamma-glutamyltransferase of the 2-nd fraction obtained from rat brain and liver are very much higher than in the first fraction and are not considerably different from the activities observed in normal animals. In contrast to brain, glutamine synthetase and gamma-glutamyltransferase activities of liver of diabetic animals do not differ from the activities observed in normal animals, both in the homogenates and in the 1-st and 2-nd fractions.</p>","PeriodicalId":76813,"journal":{"name":"Voprosy biokhimii mozga","volume":"11 ","pages":"29-34"},"PeriodicalIF":0.0000,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Rat brain and liver glutamine synthetase and gamma-glutamyltransferase activity in alloxan diabetes (IV)].\",\"authors\":\"V B Egian,&nbsp;G A Turshian,&nbsp;G E Akopian,&nbsp;S S Safrazian,&nbsp;G Kh Buniatian\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Glutamine synthetase and gamma-glutamyltransferase activities of brain and liver homogenates of rats suffering from alloxan diabetes were determined in the soluble fraction (fraction 1) and in that obtained after treatment with 0.2 percent deoxycholate (fraction 2). The results obtained indicate that the activities of these enzymes in homogenates of brain and liver of diabetic animals does not differ from that of normal animals. gamma-Glutamyltransferase activity of brain is significantly reduced (about 5 fold) in the soluble fraction while glutamine synthetase activity is not much changed. The activities of glutamine-synthetase and gamma-glutamyltransferase of the 2-nd fraction obtained from rat brain and liver are very much higher than in the first fraction and are not considerably different from the activities observed in normal animals. In contrast to brain, glutamine synthetase and gamma-glutamyltransferase activities of liver of diabetic animals do not differ from the activities observed in normal animals, both in the homogenates and in the 1-st and 2-nd fractions.</p>\",\"PeriodicalId\":76813,\"journal\":{\"name\":\"Voprosy biokhimii mozga\",\"volume\":\"11 \",\"pages\":\"29-34\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1976-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Voprosy biokhimii mozga\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Voprosy biokhimii mozga","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

测定了四氧嘧啶型糖尿病大鼠脑和肝匀浆中谷氨酰胺合成酶和γ -谷氨酰胺转移酶的活性,分别测定了可溶部分(1)和经0.2%脱氧胆酸盐处理后的脑和肝匀浆中谷氨酰胺合成酶和γ -谷氨酰胺转移酶的活性。结果表明,糖尿病大鼠脑和肝匀浆中这些酶的活性与正常动物没有差异。脑γ -谷氨酰转移酶活性在可溶性部分显著降低(约5倍),而谷氨酰胺合成酶活性变化不大。从大鼠脑和肝脏中提取的谷氨酰胺合成酶和γ -谷氨酰胺转移酶的活性远高于第一部分,与正常动物的活性无显著差异。与大脑相反,糖尿病动物肝脏的谷氨酰胺合成酶和γ -谷氨酰转移酶活性与正常动物的活性没有区别,无论是在匀浆中还是在1- 1和2- 2馏分中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Rat brain and liver glutamine synthetase and gamma-glutamyltransferase activity in alloxan diabetes (IV)].

Glutamine synthetase and gamma-glutamyltransferase activities of brain and liver homogenates of rats suffering from alloxan diabetes were determined in the soluble fraction (fraction 1) and in that obtained after treatment with 0.2 percent deoxycholate (fraction 2). The results obtained indicate that the activities of these enzymes in homogenates of brain and liver of diabetic animals does not differ from that of normal animals. gamma-Glutamyltransferase activity of brain is significantly reduced (about 5 fold) in the soluble fraction while glutamine synthetase activity is not much changed. The activities of glutamine-synthetase and gamma-glutamyltransferase of the 2-nd fraction obtained from rat brain and liver are very much higher than in the first fraction and are not considerably different from the activities observed in normal animals. In contrast to brain, glutamine synthetase and gamma-glutamyltransferase activities of liver of diabetic animals do not differ from the activities observed in normal animals, both in the homogenates and in the 1-st and 2-nd fractions.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信