球虫的适当照料和饲养:一种培养二态期球虫和波萨达斯球虫的实验室指南

Current Protocols in Microbiology Pub Date : 2020-09-07 DOI:10.1002/cpmc.113

Heather L. Mead, Marley C. Caballero Van Dyke, Bridget M. Barker

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引用次数: 11

摘要

球孢子菌病(“谷热”)是由球孢子虫和波萨达梭菌引起的。这些真菌是热二态的，在环境中在菌丝和关节分生孢子之间循环，在宿主体内转化为小球体和内生孢子。球虫可引起广泛的疾病，难以治疗。这种疾病一直在稳步增加，据估计，美国每年有35万例新感染病例。随着疾病的增加和治疗难度的增加，增加基础生物学研究和确定新的治疗方法、诊断方法和候选疫苗的需求尚未得到满足。在这里，我们描述了任何球虫实验室所需的方案，如生长、收获和储存这种二态真菌病原体的不同阶段。©2020 Wiley期刊有限公司基本方案1:液体菌丝培养物的生长和收获用于提取的替代方案1:液体菌丝培养物的大量生长和收获基本方案2:菌丝在固体培养基上的生长替代方案2:在固体培养基上维持菌丝的生长基本方案3:关节孢子的收获和定量替代方案3:关节孢子的长期储存基本方案4:寄生球体的生长和收获替代方案4:从球体中获得内生孢子基本方案5:小鼠模型的鼻内感染

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Proper Care and Feeding of Coccidioides: A Laboratorian's Guide to Cultivating the Dimorphic Stages of C. immitis and C. posadasii

Coccidioidomycosis (“Valley fever”) is caused by Coccidioides immitis and C. posadasii. These fungi are thermally dimorphic, cycling between mycelia and arthroconidia in the environment and converting into spherules and endospores within a host. Coccidioides can cause a broad spectrum of disease that can be difficult to treat. There has been a steady increase in disease, with an estimated 350,000 new infections per year in the United States. With the increase in disease and difficulty in treatment, there is an unmet need to increase research in basic biology and identify new treatments, diagnostics, and vaccine candidates. Here, we describe protocols required in any Coccidioides laboratory, such as growing, harvesting, and storing the different stages of this dimorphic fungal pathogen. © 2020 Wiley Periodicals LLC.

Basic Protocol 1: Growth and harvest of liquid mycelia cultures for extractions

Alternate Protocol 1: Large-volume growth and harvest of liquid mycelia cultures

Basic Protocol 2: Mycelial growth on solid medium

Alternate Protocol 2: Maintaining mycelial growth on solid medium

Basic Protocol 3: Harvesting and quantification of arthroconidia

Alternate Protocol 3: Long-term storage of arthroconidia

Basic Protocol 4: Parasitic spherule growth and harvest

Alternate Protocol 4: Obtaining endospores from spherules

Basic Protocol 5: Intranasal infection of murine models

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来源期刊

Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.