lncRNA MEG3-microRNA-21和内质网应激蛋白在寻常型银屑病发病中的可能作用

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zeinab Ahmed Nour, Yasmine Elwan, Yasser Nassar, Maha Fathy Elmasry, Laila Rashed, Sara Salama Ashour
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引用次数: 2

摘要

背景:银屑病是一种由遗传风险变异和环境相互作用引起的慢性炎症性免疫介导疾病。母系表达基因3 (MEG3)是一种长链非编码RNA (lncRNA),具有基因转录调控和抑制增殖的功能。MEG3与microRNA (miRNA-21)竞争,影响细胞增殖和凋亡平衡。内质网(ER)应激蛋白通过MEG3影响的未折叠蛋白反应(UPR)促进细胞存活。我们的目的是检测MEG3、miRNA-21和内质网应激蛋白在寻常型银屑病发病中的可能作用。方法:采用酶联免疫吸附法(ELISA)检测人GRP78、ATF6、caspase3组织水平。采用定量实时聚合酶链反应(qRT-PCR)检测长链非编码MEG3和miRNA 21的表达。结果:与正常皮肤相比,匀浆银屑病皮损中MEG3的表达显著下调,miRNA-21的表达显著上调,内质网应激蛋白GRP78、ATF6和caspase 3的表达均较低。miRNA 21和MEG3被确定为寻常型银屑病可能的诊断标志物。讨论:MEG3在银屑病皮损中几乎不表达,而miRNA-21的表达明显升高,但当它们相互关联时却出现了意想不到的正相关。MEG3和miRNA-21被确定为银屑病可能的诊断标志物。未分化的银屑病病变具有非常弱的UPR。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Possible Role of lncRNA MEG3-microRNA-21 and Endoplasmic Reticulum (ER) Stress Proteins in the Pathogenesis of Psoriasis Vulgaris.

Background: Psoriasis is a chronic inflammatory immune mediated disease arising from interaction between genetic risk variants and the environment. Maternally expressed gene3 (MEG3) is a long noncoding RNA (lncRNA) known for gene transcription regulation and inhibiting proliferation. MEG3 competes with microRNA (miRNA-21) influencing cell proliferation and apoptosis balance. Endoplasmic reticulum (ER) stress proteins promote cell survival via unfolded protein response (UPR) influenced by MEG3. We aimed to detect the possible role of MEG3, miRNA-21 and ER stress proteins in pathogenesis of psoriasis vulgaris.

Methods: Human GRP78, ATF6, caspase3 tissue levels were assayed by Enzyme Linked Immunosorbent Assay (ELISA). Assessment of long non-coding MEG3 and miRNA 21 expressions was done by quantitative real time polymerase chain reaction (qRT-PCR).

Results: Expression of MEG3 was significantly downregulated, while miRNA-21 was remarkably upregulated, ER stress proteins GRP78, ATF6, and caspase 3 all showed low levels in homogenized psoriatic lesions when compared to normal skin. miRNA 21 and MEG3 were identified as possible diagnostic markers for psoriasis vulgaris.

Discussion: MEG3 is barely expressed in psoriatic lesions while miRNA-21 expression is remarkably elevated but when correlated to each other there was unexpected positive correlation. MEG3 and miRNA-21 were identified as possible diagnostic markers for psoriasis. Undifferentiated psoriatic lesions have very weak UPR.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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