{"title":"苦参碱通过microRNA-299-3p/PGAM1轴诱导肝癌细胞凋亡，抑制EMT和干细胞。","authors":"BaoLin Wang, HuiHai Wang, Qin Zhao, Fei Lu, ZhenZhuang Yan, Fang Zhou, QingLun Su","doi":"10.1080/08977194.2022.2113073","DOIUrl":null,"url":null,"abstract":"<p><p>This study explored the impacts of matrine on hepatocellular carcinoma (HCC) cell growth, metastasis, epithelial-mesenchymal transition (EMT), and stemness through regulating the microRNA <i>(miR)-299-3p</i>/<i>phosphoglycerate mutase 1</i> (<i>PGAM1</i>) axis. The association between <i>miR-299-3p</i> expression with the prognosis of HCC patients was studied. <i>miR-299-3p</i> and <i>PGAM1</i> sequences were transfected into matrine-treated HCC cells, and cell proliferation, invasion, apoptosis, and stemness were detected, as well as protein expression of EMT- and stemness-related makers. The targeting relationship between <i>miR-299-3p</i> and <i>PGAM1</i> was identified. Matrine elevated <i>miR-299-3p</i> expression, repressed proliferation, invasion, and anti-apoptosis of HCC cells, and constrained EMT and stemness <i>in vitro</i>. <i>PGAM1</i> was a target of <i>miR-299-3p</i>. Repression of <i>PGAM1</i> rescued the effects of <i>miR-299-3p</i> downregulation on HCC cells. Matrine stimulates HCC cell apoptosis and represses the process of EMT and stemness through the <i>miR-299-3p</i>/<i>PGAM1</i> axis.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Matrine induces hepatocellular carcinoma apoptosis and represses EMT and stemness through microRNA-299-3p/PGAM1 axis.\",\"authors\":\"BaoLin Wang, HuiHai Wang, Qin Zhao, Fei Lu, ZhenZhuang Yan, Fang Zhou, QingLun Su\",\"doi\":\"10.1080/08977194.2022.2113073\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study explored the impacts of matrine on hepatocellular carcinoma (HCC) cell growth, metastasis, epithelial-mesenchymal transition (EMT), and stemness through regulating the microRNA <i>(miR)-299-3p</i>/<i>phosphoglycerate mutase 1</i> (<i>PGAM1</i>) axis. The association between <i>miR-299-3p</i> expression with the prognosis of HCC patients was studied. <i>miR-299-3p</i> and <i>PGAM1</i> sequences were transfected into matrine-treated HCC cells, and cell proliferation, invasion, apoptosis, and stemness were detected, as well as protein expression of EMT- and stemness-related makers. The targeting relationship between <i>miR-299-3p</i> and <i>PGAM1</i> was identified. Matrine elevated <i>miR-299-3p</i> expression, repressed proliferation, invasion, and anti-apoptosis of HCC cells, and constrained EMT and stemness <i>in vitro</i>. <i>PGAM1</i> was a target of <i>miR-299-3p</i>. Repression of <i>PGAM1</i> rescued the effects of <i>miR-299-3p</i> downregulation on HCC cells. Matrine stimulates HCC cell apoptosis and represses the process of EMT and stemness through the <i>miR-299-3p</i>/<i>PGAM1</i> axis.</p>\",\"PeriodicalId\":12782,\"journal\":{\"name\":\"Growth factors\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Growth factors\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/08977194.2022.2113073\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Growth factors","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/08977194.2022.2113073","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Matrine induces hepatocellular carcinoma apoptosis and represses EMT and stemness through microRNA-299-3p/PGAM1 axis.
This study explored the impacts of matrine on hepatocellular carcinoma (HCC) cell growth, metastasis, epithelial-mesenchymal transition (EMT), and stemness through regulating the microRNA (miR)-299-3p/phosphoglycerate mutase 1 (PGAM1) axis. The association between miR-299-3p expression with the prognosis of HCC patients was studied. miR-299-3p and PGAM1 sequences were transfected into matrine-treated HCC cells, and cell proliferation, invasion, apoptosis, and stemness were detected, as well as protein expression of EMT- and stemness-related makers. The targeting relationship between miR-299-3p and PGAM1 was identified. Matrine elevated miR-299-3p expression, repressed proliferation, invasion, and anti-apoptosis of HCC cells, and constrained EMT and stemness in vitro. PGAM1 was a target of miR-299-3p. Repression of PGAM1 rescued the effects of miR-299-3p downregulation on HCC cells. Matrine stimulates HCC cell apoptosis and represses the process of EMT and stemness through the miR-299-3p/PGAM1 axis.
期刊介绍：
Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications
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