大鼠气管肌相关神经末梢突触释放蛋白的分布

IF 3.2 4区医学 Q2 NEUROSCIENCES

Autonomic Neuroscience-Basic & Clinical Pub Date : 2023-01-01 DOI:10.1016/j.autneu.2022.103042

Hisae Moriai , Takuya Yokoyama , Sayed Sharif Abdali , Nobuaki Nakamuta , Yoshio Yamamoto

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引用次数: 0

摘要

使用大鼠气管的整体支架制剂在平滑肌相关感觉神经末梢中检测突触释放蛋白的免疫组织化学定位。在气管肌中发现了对Na+-K+-ATP酶、α3-亚基具有免疫反应性的植物状平滑肌相关神经末梢。在这些末端的末端部分检测到VGLUT1、突触蛋白1、t-SNARE蛋白（SNAP25和突触新蛋白1）、v-SNARE蛋白质（VAMP1和VAMP2）和突触前活性区相关蛋白（短笛）。这些结果表明，平滑肌相关神经末梢分泌谷氨酸来调节肺收缩反射中的感觉运动功能。

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Distribution of proteins for synaptic release in nerve endings associated with the trachealis muscle of rats

The immunohistochemical localization of proteins for synaptic release was examined in smooth muscle-associated sensory nerve endings using whole-mount preparations of the rat trachea. Plant-like smooth muscle-associated nerve endings with immunoreactivity for Na⁺-K⁺-ATPase, α₃-subunit were identified in the trachealis muscle. VGLUT1, synapsin1, t-SNARE proteins (SNAP25 and syntaxin1), v-SNARE proteins (VAMP1 and VAMP2), and a presynaptic active zone-related protein (piccolo) were detected in the terminal parts of these endings. These results suggest that smooth muscle-associated nerve endings secrete glutamate to modulate sensorimotor functions in the lung deflation reflex.

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来源期刊

Autonomic Neuroscience-Basic & Clinical 医学-神经科学

CiteScore

5.80

自引率

7.40%

发文量

审稿时长

66 days

期刊介绍： This is an international journal with broad coverage of all aspects of the autonomic nervous system in man and animals. The main areas of interest include the innervation of blood vessels and viscera, autonomic ganglia, efferent and afferent autonomic pathways, and autonomic nuclei and pathways in the central nervous system. The Editors will consider papers that deal with any aspect of the autonomic nervous system, including structure, physiology, pharmacology, biochemistry, development, evolution, ageing, behavioural aspects, integrative role and influence on emotional and physical states of the body. Interdisciplinary studies will be encouraged. Studies dealing with human pathology will be also welcome.