脱细胞真皮基质修复大鼠坐骨神经缺损的实验研究。

IF 1 4区医学 Q3 ORTHOPEDICS

Journal of Plastic Surgery and Hand Surgery Pub Date : 2023-02-01 DOI:10.1080/2000656X.2022.2152824

Fatih Ceran, Ozgur Pilanci, Asuman Ozel, Gul Ilbay, Rukiye Karabacak, Mehmet Kanter, Konuralp Ilbay, Samet Vasfi Kuvat

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引用次数: 1

摘要

背景:在神经组织缺损患者中，自体神经移植是治疗的标准方法。自体神经移植的替代品已经引起了重建外科医生的注意。本研究报道了脱细胞真皮基质(ADM)对实验性大鼠坐骨神经缺损模型的修复效果。方法:36只sd大鼠随机分为5组:第1组:对照组，第2组:阴性对照组(n = 6)，第3组:自体神经移植组(n = 10)，第4组:供区灌管组(n = 10);第5组:神经移植物内插ADM组(n = 10)。于第3、6个月测定各组动物的电生理功能、腓肠肌重量和组织形态学。结果:与对照组相比，3、4、5组的肌肉复合动作电位明显降低。4组第6个月腓肠肌比值(GCR)值低于3组和5组，5组组织学评分和髓鞘轴突计数高于3组和4组，差异有统计学意义。结论:本研究结果表明，在神经移植物周围包裹ADM可获得更好的神经愈合效果。

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Use of acellular dermal matrix in peripheral nerve reconstruction: an experimental study on rat sciatic nerve defect.

Background: In patients with nerve tissue defects, the use of autologous nerve grafts is the standard method of treatment. Alternatives to autologous, nerve grafts have attracted the attention of reconstructive surgeons. In this study, the results of nerve repairs using acellular dermal matrix (ADM) in an experimental rat sciatic nerve defect model are presented.

Methods: Thirty-six Sprague-Dawley rats were randomized into 5 groups: Group 1: control group, Group 2: negative control group (n = 6), Group 3: autologous nerve graft group (n = 10), Group 4: donor site entubulated with ADM group (n = 10); and Group 5: nerve graft entubulated with ADM group (n = 10). The animals in each group were evaluated for electrophysiologic functions, gastrocnemius muscle weight and histomorphology on the 3rd and 6th month.

Results: The compound muscle action potential was observed to be distinctly lower in Groups 3, 4 and 5 in comparison to the control group. In Group 4, the gastrocnemius ratio (GCR) values on the 6th month were statistically significantly lower than the GCR values in Group 3 and Group 5, The histological scores and myelinated axonal counts in Group 5 were statistically significantly higher than the values in Group 3 and Group 4.

Conclusion: The results of this study showed that wrapping ADM around nerve grafts resulted in better outcomes with respect to nerve healing.

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来源期刊

Journal of Plastic Surgery and Hand Surgery ORTHOPEDICS-SURGERY

CiteScore

2.10

自引率

0.00%

发文量

108

审稿时长

6-12 weeks

期刊介绍： The purpose of the Journal of Plastic Surgery and Hand Surgery is to serve as an international forum for plastic surgery, hand surgery and related research. Interest is focused on original articles on basic research and clinical evaluation. The scope of the journal comprises: • Articles concerning operative methods and follow-up studies • Research articles on subjects related to plastic and hand surgery • Articles on cranio-maxillofacial surgery, including cleft lip and palate surgery. Extended issues are published occasionally, dealing with special topics such as microvascular surgery, craniofacial surgery, or burns. Supplements, usually doctoral theses, may also be published. The journal is published for the Acta Chirurgica Scandinavica society and sponsored by the Key Foundation, Sweden. The journal was previously published as Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery.