自身免疫性甲状腺炎中RAB8A和RAP1A的全血DNA甲基化:来自不同水碘区人群碘暴露的证据和验证

IF 2.2 4区医学 Q3 ENVIRONMENTAL SCIENCES

International Journal of Environmental Health Research Pub Date : 2024-08-01 Epub Date: 2023-11-14 DOI:10.1080/09603123.2023.2280148

Hongmei Shen, Jinjin Liu, Yun Chen, Bingxuan Ren, Zheng Zhou, Meihui Jin, Lingbo Wang, Yanhong He, Fan Li, Baoxiang Li, Mengxue Du

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引用次数: 0

摘要

我们的研究旨在鉴定和验证AIT患者中G蛋白相关甲基化基因，同时研究不同水碘区暴露于碘的AIT患者中这些基因。根据水中碘(MWI)浓度中位数对不同区域进行分类:碘强化区(IFA, MWI100µg/L)。我们研究了176例AIT病例和176例对照，其中IFA、IAA和IEA分别有89对、40对和47对。使用Illumina Human Methylation 850k BeadChip，我们鉴定了候选的甲基化基因。MethylTargetTM和QRT-PCR验证了DNA甲基化和mRNA表达。结果显示，176例AIT患者均存在低甲基化和高表达的RAB8A和RAP1A。RAB8A的CpG位点主要在IFA和IEA中低甲基化，而RAP1A的CpG位点主要在IEA中低甲基化。本研究强调了水碘暴露如何影响AIT患者RAB8A和RAP1A甲基化。

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The whole blood DNA methylation of RAB8A and RAP1A in autoimmune thyroiditis: evidence and validation of iodine exposure in a population from different water iodine areas.

Our study aimed to identify and verify G protein-related methylated genes in AIT patients, while also investigate those genes in AIT patients exposed to iodine in different water iodine areas. Different areas were classified by median water iodine (MWI) concentrations: Iodine-Fortified Areas (IFA, MWI<10µg/L), Iodine-Adequate Areas (IAA, 40≤MWI≤100 µg/L), and Iodine-Excessive Areas (IEA, MWI>100 µg/L). We studied 176 AIT cases and 176 controls, with 89, 40, and 47 pairs in IFA, IAA, and IEA, respectively. Using the Illumina Human Methylation 850k BeadChip, we identified candidate methylated genes. MethylTarget^TM and QRT-PCR validated DNA methylation and mRNA expression. Results showed hypomethylation and high expression of RAB8A and RAP1A in all 176 AIT cases. RAB8A's CpG sites were mainly hypomethylated in IFA and IEA, while RAP1A's sites were primarily hypomethylated in IEA. This study underscores how water iodine exposure may influence RAB8A and RAP1A methylation in AIT.

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来源期刊

International Journal of Environmental Health Research 医学-公共卫生、环境卫生与职业卫生

CiteScore

6.70

自引率

3.10%

发文量

134

审稿时长

>12 weeks

期刊介绍： International Journal of Environmental Health Research ( IJEHR ) is devoted to the rapid publication of research in environmental health, acting as a link between the diverse research communities and practitioners in environmental health. Published articles encompass original research papers, technical notes and review articles. IJEHR publishes articles on all aspects of the interaction between the environment and human health. This interaction can broadly be divided into three areas: the natural environment and health – health implications and monitoring of air, water and soil pollutants and pollution and health improvements and air, water and soil quality standards; the built environment and health – occupational health and safety, exposure limits, monitoring and control of pollutants in the workplace, and standards of health; and communicable diseases – disease spread, control and prevention, food hygiene and control, and health aspects of rodents and insects. IJEHR is published in association with the International Federation of Environmental Health and includes news from the Federation of international meetings, courses and environmental health issues.