牙龈卟啉单胞菌蛋白质相互作用组图谱揭示了九型分泌系统 PorQ-Z 复合物的形成过程。

IF 2.8 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Molecular Oral Microbiology Pub Date : 2023-02-01 Epub Date: 2022-08-03 DOI:10.1111/omi.12383
Dhana G Gorasia, Paul D Veith, Eric C Reynolds
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引用次数: 0

摘要

牙龈卟啉单胞菌是一种厌氧革兰阴性人类口腔病原体,与较严重的牙周病高度相关。牙龈卟啉单胞菌利用 IX 型分泌系统(T9SS)将包括多种毒力因子在内的 30 ∼ 30 种货物蛋白运输到细胞表面。T9SS是一个多蛋白系统,由至少20个蛋白组成,最近我们对这些成分的蛋白相互作用组进行了表征。与 T9SS 类似,几乎所有生物过程都是通过蛋白质-蛋白质相互作用(PPIs)介导的。因此,绘制 PPIs 图谱对于了解牙龈炎杆菌中许多蛋白质的生物功能非常重要。在本文中,我们提供了 1000 多种牙龈杆菌蛋白质的原生迁移图谱。通过使用 T9SS,我们证明我们的数据集是鉴定新型蛋白质相互作用的有用资源。利用该数据集以及对 T9SS P. gingivalis 突变体的进一步分析,我们发现了 T9SS PorQ-Z 复合物形成的新机理。该数据集是研究牙龈炎杆菌的宝贵资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protein interactome mapping of Porphyromonas gingivalis provides insights into the formation of the PorQ-Z complex of the type IX secretion system.

Porphyromonas gingivalis is an anaerobic Gram-negative human oral pathogen highly associated with the more severe forms of periodontal disease. Porphyromonas gingivalis utilises the type IX secretion system (T9SS) to transport ∼30 cargo proteins, including multiple virulence factors, to the cell surface. The T9SS is a multiprotein system consisting of at least 20 proteins, and recently, we characterised the protein interactome of these components. Similar to the T9SS, almost all biological processes are mediated through protein-protein interactions (PPIs). Therefore, mapping PPIs is important to understand the biological functions of many proteins in P. gingivalis. Herein, we provide native migration profiles of over 1000 P. gingivalis proteins. Using the T9SS, we demonstrate that our dataset is a useful resource for identifying novel protein interactions. Using this dataset and further analysis of T9SS P. gingivalis mutants, we discover new mechanistic insights into the formation of the PorQ-Z complex of the T9SS. This dataset is a valuable resource for studies of P. gingivalis.

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来源期刊
Molecular Oral Microbiology
Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY
CiteScore
6.50
自引率
5.40%
发文量
46
审稿时长
>12 weeks
期刊介绍: Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.
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