植物多酚对紫外线照射下角质形成细胞DNA修复激活和细胞死亡抑制的影响。

IF 2.2 4区医学 Q3 GERIATRICS & GERONTOLOGY

Rejuvenation research Pub Date : 2023-02-01 DOI:10.1089/rej.2022.0031

Alla I Potapovich, Tatyana V Kostyuk, Tatyana V Shman, Tatyana I Ermilova, Tatyana G Shutava, Vladimir A Kostyuk

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引用次数: 0

摘要

本文研究了植物多酚化合物(PPs)对培养的人HaCaT角质形成细胞对紫外线辐射(UV-C)的影响。获得的实验数据表明，在UV-C暴露后立即添加PPs具有细胞保护作用。PPs的效率依次为:阿沙星素≥水飞蓟宾>槲皮素。还研究了PPs对组蛋白H2AX磷酸化和角质形成细胞细胞核单链DNA断裂数量的影响。通过彗星实验和γ - h2ax染色，以及荧光显微镜，我们发现PPs可以减少UV-C照射下角质形成细胞细胞核中的DNA损伤。由此可见，PPs可减轻紫外线对皮肤细胞的破坏作用，激活基因损伤的修复过程。

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DNA Repair Activation and Cell Death Suppression by Plant Polyphenols in Keratinocytes Exposed to Ultraviolet Irradiation.

This work investigated effects of plant polyphenolic compounds (PPs) on responses of cultured human HaCaT keratinocytes to ultraviolet radiation in the C range (UV-C). The experimental data obtained indicate a cytoprotective effect of PPs added immediately after UV-C exposure. The efficiency of PPs was lowered in the following order: acacetin ≥ silybin > quercetin. The influence of PPs on phosphorylation of histone H2AX and the number of single-strand DNA breaks in the nuclei of keratinocytes were also studied. Using the comet assay and γH2AX staining, followed by fluorescence microscopy, it has been established that PPs can reduce DNA damage in the nuclei of keratinocytes exposed to UV-C. It is concluded that PPs can diminish the destructive effect of UV radiation on skin cells, activating the process of repairing genetic damage.

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来源期刊

Rejuvenation research 医学-老年医学

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Rejuvenation Research publishes cutting-edge, peer-reviewed research on rejuvenation therapies in the laboratory and the clinic. The Journal focuses on key explorations and advances that may ultimately contribute to slowing or reversing the aging process, and covers topics such as cardiovascular aging, DNA damage and repair, cloning, and cell immortalization and senescence. Rejuvenation Research coverage includes: Cell immortalization and senescence Pluripotent stem cells DNA damage/repair Gene targeting, gene therapy, and genomics Growth factors and nutrient supply/sensing Immunosenescence Comparative biology of aging Tissue engineering Late-life pathologies (cardiovascular, neurodegenerative and others) Public policy and social context.