环状RNA CSPP1通过microRNA-493-5p靶向RAC1，从而促进肾细胞癌的发生和Warburg效应。

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Acta biochimica Polonica Pub Date : 2023-09-18 DOI:10.18388/abp.2020_6299

Dong Zhang, XiaoJie Yang, QiDong Luo, DeLai Fu, HongLiang Li, Peng Zhang, Chong Tie

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引用次数: 0

摘要

环状核糖核酸（circRNAs）在肾细胞癌（RCC）中发挥调节作用。本研究的目的是弄清circ-CSPP1在RCC中的作用和分子机制。结果表明circ-CSPP1在RCC中的表达增强。下调circ-CSPP1抑制了RCC细胞的增殖、迁移、侵袭和Warburg效应（有氧糖酵解），但加速了细胞凋亡。萤光素酶活性测定显示circ-CSPP1可以作为miR-493-5p的内源性海绵。miR-493-5p的升高抑制了RCC的进展。生物信息学网站starBase证实，ras相关的C3肉毒杆菌毒素底物1（RAC1）是miR-493-5p的靶基因。Circ-CSPP1通过吸收miR-493-5p上调RAC1，而升高RAC1可以逆转下调Circ-CSP1对RCC细胞的影响。总之，circ-CSPP1被鉴定为一种新的RCC促进RNA，可作为RCC治疗的潜在治疗靶点。

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Circular RNA CSPP1 motivates renal cell carcinoma carcinogenesis and the Warburg effect by targeting RAC1 through microRNA-493-5p.

Circular RNAs (circRNAs) take on regulatory roles in renal cell carcinoma (RCC). The research's goal was to figure out circ-CSPP1's role and molecular mechanism in RCC. The results clarified that circ-CSPP1 expression was enhanced in RCC. Down-regulating circ-CSPP1 refrained the proliferation, migration, invasion, and Warburg effect (aerobic glycolysis), but accelerated apoptosis of RCC cells. The luciferase activity assay exhibited that circ-CSPP1 could perform as an endogenous sponge for miR-493-5p. Elevating miR-493-5p repressed RCC progression. The bioinformatics website starBase confirmed that ras-related C3 botulinum toxin substrate 1 (RAC1) was a target gene of miR-493-5p. Circ-CSPP1 up-regulated RAC1 by sponging miR-493-5p, and elevating RAC1 could turn around the effect of down-regulating circ-CSPP1 on RCC cells. Taken together, circ-CSPP1 is identified as a novel RCC-promoting RNA that could serve as a latent therapeutic target for RCC therapy.

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来源期刊

Acta biochimica Polonica 生物-生化与分子生物学

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Acta Biochimica Polonica is a journal covering enzymology and metabolism, membranes and bioenergetics, gene structure and expression, protein, nucleic acid and carbohydrate structure and metabolism.