小鼠细菌性病原体衣原体在研究小鼠菌落中的再次出现。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Noah Mishkin, Rodolfo J Ricart Arbona, Sebastian E Carrasco, Samira Lawton, Kenneth S Henderson, Panagiota Momtsios, Ira M Sigar, Kyle H Ramsey, Christopher Cheleuitte-Nieves, Sebastien Monette, Neil S Lipman
{"title":"小鼠细菌性病原体衣原体在研究小鼠菌落中的再次出现。","authors":"Noah Mishkin,&nbsp;Rodolfo J Ricart Arbona,&nbsp;Sebastian E Carrasco,&nbsp;Samira Lawton,&nbsp;Kenneth S Henderson,&nbsp;Panagiota Momtsios,&nbsp;Ira M Sigar,&nbsp;Kyle H Ramsey,&nbsp;Christopher Cheleuitte-Nieves,&nbsp;Sebastien Monette,&nbsp;Neil S Lipman","doi":"10.30802/AALAS-CM-22-000045","DOIUrl":null,"url":null,"abstract":"<p><p><i>Chlamydia muridarum</i> (Cm) was detected in 2 colonies of mice with lymphoplasmacytic pulmonary infiltrates by using PCR and immunohistochemistry. This discovery was unexpected, as Cm infection had not been reported in laboratory mice since the 1940s. A Cm specific PCR assay was developed and testing implemented for the resident colonies of 8 vivaria from 3 academic institutions, 58 incoming mouse shipments from 39 academic institutions, and mice received from 55 commercial breeding colonies (4 vendors). To estimate Cm's global prevalence in research colonies, a database containing 11,387 metagenomic fecal microbiota samples from 120 institutions and a cohort of 900 diagnostic samples from 96 institutions were examined. Results indicate significant prevalence among academic institutions, with Cm detected in 63% of soiled bedding sentinels from 3 institutions; 33% of incoming mouse shipments from 39 academic institutions; 14% of 120 institutions submitting microbiota samples; and 16% of the diagnostic sample cohort. All samples from commercial breeding colonies were negative. In addition, naïve NOD. Cg-<i>Prkdc</i><sup>scid</sup>Il2rg<sup>tm1Wjl</sup>/SzJ (NSG) mice exposed to Cm-shedding mice and/or their soiled bedding developed clinical disease at 21 to 28 d after exposure. These mice had a moderate-to-severe histiocytic and neutro- philic bronchointerstitial pneumonia, with their respiratory epithelium demonstrating inclusions, chlamydial major outer membrane protein immunostaining, and hybridization with a Cm reference sequence (GenBank accession no. U68436). Cm was isolated from lungs, cecum, and feces of a Cm-infected NSG mouse by using HeLa 229 cells. The considerable prevalence of Cm is likely due to widespread global interinstitutional distribution of unique mouse strains and failure to recognize that some of these mice were from enzootically infected colonies. Given that experimental Cm colonization of mice results in a robust immune response and, on occasion, pathology, natural infection may confound experimental results. Therefore, Cm should be excluded and eradicated from enzootically infected mouse colonies.</p>","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413529/pdf/cm2022000230.pdf","citationCount":"4","resultStr":"{\"title\":\"Reemergence of the Murine Bacterial Pathogen <i>Chlamydia muridarum</i> in Research Mouse Colonies.\",\"authors\":\"Noah Mishkin,&nbsp;Rodolfo J Ricart Arbona,&nbsp;Sebastian E Carrasco,&nbsp;Samira Lawton,&nbsp;Kenneth S Henderson,&nbsp;Panagiota Momtsios,&nbsp;Ira M Sigar,&nbsp;Kyle H Ramsey,&nbsp;Christopher Cheleuitte-Nieves,&nbsp;Sebastien Monette,&nbsp;Neil S Lipman\",\"doi\":\"10.30802/AALAS-CM-22-000045\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Chlamydia muridarum</i> (Cm) was detected in 2 colonies of mice with lymphoplasmacytic pulmonary infiltrates by using PCR and immunohistochemistry. This discovery was unexpected, as Cm infection had not been reported in laboratory mice since the 1940s. A Cm specific PCR assay was developed and testing implemented for the resident colonies of 8 vivaria from 3 academic institutions, 58 incoming mouse shipments from 39 academic institutions, and mice received from 55 commercial breeding colonies (4 vendors). To estimate Cm's global prevalence in research colonies, a database containing 11,387 metagenomic fecal microbiota samples from 120 institutions and a cohort of 900 diagnostic samples from 96 institutions were examined. Results indicate significant prevalence among academic institutions, with Cm detected in 63% of soiled bedding sentinels from 3 institutions; 33% of incoming mouse shipments from 39 academic institutions; 14% of 120 institutions submitting microbiota samples; and 16% of the diagnostic sample cohort. All samples from commercial breeding colonies were negative. In addition, naïve NOD. Cg-<i>Prkdc</i><sup>scid</sup>Il2rg<sup>tm1Wjl</sup>/SzJ (NSG) mice exposed to Cm-shedding mice and/or their soiled bedding developed clinical disease at 21 to 28 d after exposure. These mice had a moderate-to-severe histiocytic and neutro- philic bronchointerstitial pneumonia, with their respiratory epithelium demonstrating inclusions, chlamydial major outer membrane protein immunostaining, and hybridization with a Cm reference sequence (GenBank accession no. U68436). Cm was isolated from lungs, cecum, and feces of a Cm-infected NSG mouse by using HeLa 229 cells. The considerable prevalence of Cm is likely due to widespread global interinstitutional distribution of unique mouse strains and failure to recognize that some of these mice were from enzootically infected colonies. Given that experimental Cm colonization of mice results in a robust immune response and, on occasion, pathology, natural infection may confound experimental results. Therefore, Cm should be excluded and eradicated from enzootically infected mouse colonies.</p>\",\"PeriodicalId\":10659,\"journal\":{\"name\":\"Comparative medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2022-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413529/pdf/cm2022000230.pdf\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.30802/AALAS-CM-22-000045\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.30802/AALAS-CM-22-000045","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 4

摘要

采用PCR和免疫组化方法,在2个淋巴浆细胞性肺浸润小鼠中检测到muridarum (Cm)衣原体。这一发现是出乎意料的,因为自20世纪40年代以来,在实验室小鼠中没有报道过Cm感染。建立了Cm特异性PCR检测方法,并对来自3个学术机构的8个活体菌落、来自39个学术机构的58只来料小鼠以及来自55个商业繁殖菌落(4个供应商)的小鼠进行了检测。为了估计Cm在研究菌落中的全球患病率,研究人员检查了一个包含来自120个机构的11,387个宏基因组粪便微生物群样本的数据库和来自96个机构的900个诊断样本的队列。研究结果表明,Cm在学术机构中普遍存在,3所院校的脏床上用品中检测到Cm的比例为63%;来自39个学术机构的33%的鼠标出货量;120个机构中有14%提交了微生物群样本;16%的诊断样本队列。所有商业繁殖群体的样本均为阴性。另外,naïve NOD。Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG)小鼠暴露于cm脱落小鼠和/或其污染的被褥后21至28 d出现临床疾病。这些小鼠患有中度至重度组织细胞性和嗜中性支气管间质性肺炎,其呼吸上皮显示包涵体,衣原体主要外膜蛋白免疫染色,并与Cm参考序列杂交(GenBank登录号:U68436)。用HeLa 229细胞从感染Cm的NSG小鼠的肺、盲肠和粪便中分离Cm。Cm的大量流行可能是由于全球范围内广泛分布的独特小鼠品系,以及未能认识到其中一些小鼠来自地方性感染菌落。鉴于实验小鼠的Cm定植导致强大的免疫反应,并且有时病理,自然感染可能混淆实验结果。因此,从动物感染的小鼠菌落中应排除和根除Cm。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Reemergence of the Murine Bacterial Pathogen Chlamydia muridarum in Research Mouse Colonies.

Chlamydia muridarum (Cm) was detected in 2 colonies of mice with lymphoplasmacytic pulmonary infiltrates by using PCR and immunohistochemistry. This discovery was unexpected, as Cm infection had not been reported in laboratory mice since the 1940s. A Cm specific PCR assay was developed and testing implemented for the resident colonies of 8 vivaria from 3 academic institutions, 58 incoming mouse shipments from 39 academic institutions, and mice received from 55 commercial breeding colonies (4 vendors). To estimate Cm's global prevalence in research colonies, a database containing 11,387 metagenomic fecal microbiota samples from 120 institutions and a cohort of 900 diagnostic samples from 96 institutions were examined. Results indicate significant prevalence among academic institutions, with Cm detected in 63% of soiled bedding sentinels from 3 institutions; 33% of incoming mouse shipments from 39 academic institutions; 14% of 120 institutions submitting microbiota samples; and 16% of the diagnostic sample cohort. All samples from commercial breeding colonies were negative. In addition, naïve NOD. Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice exposed to Cm-shedding mice and/or their soiled bedding developed clinical disease at 21 to 28 d after exposure. These mice had a moderate-to-severe histiocytic and neutro- philic bronchointerstitial pneumonia, with their respiratory epithelium demonstrating inclusions, chlamydial major outer membrane protein immunostaining, and hybridization with a Cm reference sequence (GenBank accession no. U68436). Cm was isolated from lungs, cecum, and feces of a Cm-infected NSG mouse by using HeLa 229 cells. The considerable prevalence of Cm is likely due to widespread global interinstitutional distribution of unique mouse strains and failure to recognize that some of these mice were from enzootically infected colonies. Given that experimental Cm colonization of mice results in a robust immune response and, on occasion, pathology, natural infection may confound experimental results. Therefore, Cm should be excluded and eradicated from enzootically infected mouse colonies.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信