两种不同来源Rag2基因敲除小鼠免疫表型的比较

IF 2.7 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Yu Jeong Roh, Jeong Eun Gong, Ji Eun Kim, You Jeong Jin, Hee Jin Song, Ayun Seol, Jumin Park, Yong Lim, Dae Youn Hwang
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引用次数: 0

摘要

背景:重组激活基因2 (Rag2)敲除(KO)小鼠被广泛应用于各个研究领域,包括疫苗开发、移植研究和造血研究,但很少有研究对它们的表型进行比较。本研究考察了不同来源的Rag2 KO小鼠的免疫表型是否存在差异。特别比较了C57BL/6-Rag2em1hwl/Korl (Rag2/Korl KO)和b6 . cg - rag2tm1.1 . 1cgn /J (Rag2/ jko)小鼠脾脏和胸腺器官重量、组织结构、T细胞亚群和B细胞亚群的变化。结果:Rag2/Korl和Rag2/ jko小鼠的脾脏和胸腺的重量与野生型(WT)小鼠相比有相似的减少,即使其他器官保持相同的重量。Rag2/Korl组与Rag2/J KO组在白细胞(WBC)、淋巴细胞(LYM)、红细胞分布宽度(RDW)、血小板(PLT)等指标上有轻微差异。此外,与WT小鼠相比,两种Rag2 KO小鼠的脾脏白髓和胸腺皮质区均减少。另一方面,在Rag2/Korl组和Rag2/J KO组中,WT和Rag2 KO小鼠的CD8+ T和B细胞亚群数量存在显著差异,而CD4+ T亚群在两组中保持相似。结论:Rag2/Korl和Rag2/ jko小鼠在脾脏和胸腺中表现出相似的免疫表型,除了CD8+ T和B细胞亚群的数量不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Comparison of immunophenotypes between Rag2 knockout mice derived from two different sources.

Comparison of immunophenotypes between Rag2 knockout mice derived from two different sources.

Comparison of immunophenotypes between Rag2 knockout mice derived from two different sources.

Comparison of immunophenotypes between Rag2 knockout mice derived from two different sources.

Background: Recombination activating gene2 (Rag2) knockout (KO) mice are used widely in various research fields, including vaccine development, transplantation studies, and hematopoiesis research, but few studies have compared their phenotypes. This study examined whether there were differences in the immunophenotypes between Rag2 KO mice derived from different sources. In particular, the changes in the organ weight, histological structure, and subpopulation of T and B cells were compared in the spleen and thymus of C57BL/6-Rag2em1hwl/Korl (Rag2/Korl KO) and B6.Cg-Rag2tm1.1Cgn/J (Rag2/J KO) mice.

Results: The weight of the spleen and thymus similarly decreased in the Rag2/Korl and Rag2/J KO mice compared to their wild type (WT) mice, even though the other organs were kept at the same weight. A slight difference between the Rag2/Korl and Rag2/J KO group were detected in the number of white blood cells (WBC), lymphocytes (LYM), red cell distribution width (RDW), and platelets (PLT). In addition, the white pulp of the spleen and the cortex region of the thymus decreased in both Rag2 KO mice compared to WT mice. On the other hand, significant differences in the number of CD8+ T and B cell subpopulations between WT and Rag2 KO mice were observed between Rag2/Korl and Rag2/J KO group, while the CD4+ T subpopulation was maintained similarly in both groups.

Conclusions: These results suggest that Rag2/Korl and Rag2/J KO mice exhibit similar immunophenotypes in the spleen and thymus except for the differences in the number of CD8+ T and B cell subpopulations.

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来源期刊
CiteScore
4.40
自引率
0.00%
发文量
32
审稿时长
8 weeks
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