唾液样本中胃蛋白酶浓度随储存时间的变化。

IF 2.5 4区医学 Q1 AUDIOLOGY & SPEECH-LANGUAGE PATHOLOGY

Journal of Voice Pub Date : 2025-05-01 DOI:10.1016/j.jvoice.2022.12.010

Hee Jin Kang , Joo Kyung Noh , Min Kyeong Lee , Seon Rang Woo , Jung Min Park , Young Chan Lee , Seong-Gyu Ko , Young-Gyu Eun

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引用次数: 0

摘要

目的：确定唾液样本中胃蛋白酶的浓度是否会随着样本储存时间的延长而发生变化：确定唾液样本中胃蛋白酶的浓度是否会随样本储存时间的延长而发生变化：本研究纳入了 48 名疑似喉咽反流患者。收集每位患者的唾液样本，并将每个样本分成六份，储存不同的时间。使用酶联免疫吸附法测定胃蛋白酶的浓度。将立即测量的胃蛋白酶浓度与储存 1 周、2 周、1 个月、3 个月和 6 个月后测量的浓度进行比较：结果：立即分析的样本与收集唾液 1 周、2 周、1 个月和 3 个月后分析的样本之间未发现胃蛋白酶浓度有明显差异。立即分析的样本与采集唾液 6 个月后分析的样本中的胃蛋白酶浓度存在明显差异：结论：唾液样本中的胃蛋白酶浓度在采集唾液后立即测量和采集唾液 3 个月后测量的浓度之间没有明显差异，但在采集唾液 6 个月后测量的浓度有明显差异。

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Changes of Pepsin Concentration in Saliva Sample According to Storage Period

Objectives

To determine whether the concentration of pepsin in the saliva sample changes according to the storage period of the sample.

Methods

Forty eight patients with suspected laryngopharyngeal reflux were included in this study. Saliva samples were collected from each patient and each sample divided into six and stored for different period of time. Pepsin concentration was measured using enzyme-linked immunosorbent assay. A comparison was made between the pepsin concentration measured immediately and the concentration measured after storage for 1 week, 2 weeks, 1 month, 3 months, and 6 months.

Results

No significant difference in pepsin concentrations were detected between the sample analyzed immediately and those analyzed at 1 week, 2 weeks, 1 month, and 3 months after saliva collection. A significant difference in pepsin concentration was observed in the sample analyzed immediately and the sample analyzed 6 months after saliva collection.

Conclusions

Pepsin concentration in saliva samples did not demonstrate a significant difference between the concentration measured immediately and the concentration measured 3 months after saliva collection, although a significant difference was observed in the concentration measured 6 months after collection.

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来源期刊

Journal of Voice 医学-耳鼻喉科学

CiteScore

4.00

自引率

13.60%

发文量

395

审稿时长

59 days

期刊介绍： The Journal of Voice is widely regarded as the world''s premiere journal for voice medicine and research. This peer-reviewed publication is listed in Index Medicus and is indexed by the Institute for Scientific Information. The journal contains articles written by experts throughout the world on all topics in voice sciences, voice medicine and surgery, and speech-language pathologists'' management of voice-related problems. The journal includes clinical articles, clinical research, and laboratory research. Members of the Foundation receive the journal as a benefit of membership.