应用ATP生物发光法评价口腔链球菌。

IF 0.9 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocontrol science Pub Date : 2022-01-01 DOI:10.4265/bio.27.229

Akari Watanabe, Naofumi Tamaki, Kenji Yokota, Susumu Kokeguchi, Hiro-O Ito, Miwa Matsuyama

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引用次数: 0

摘要

研究口腔链球菌数量与ATP生物发光测定结果的统计学相关性，并与链球菌平板计数和口腔细菌定量系统的结果进行比较。由于在口腔细菌定量系统中检测的7种口腔链球菌中，ATP (RLU)与细菌数量之间存在显著相关性，因此ATP可以反映口腔卫生状况。然而，使用这种分析，观察到可能很难正确评估形成聚集体的细菌。此外，即使少量细菌(低于105 CFU/mL)无法通过口腔细菌定量系统测量，也可以通过ATP生物发光法估计。提示该方法可用于口腔清洁效果的定量评价。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Use of ATP Bioluminescence Assay to Evaluate Oral Streptococci.

The statistical correlation between the number of oral streptococci and the results of ATP bioluminescence assay was examined and compared with the results from Streptococcus plate counts and an oral bacteria quantification system. Because a significant correlation was found between ATP (RLU) and the number of bacteria in the oral bacteria quantification system for all seven types of oral streptococci examined, ATP would reflect a conditions of oral hygiene. However, using this assay, it was observed it may be difficult to correctly evaluate bacteria that form aggregates. Furthermore, even a small number of bacteria (below 10⁵ CFU/mL) , which cannot be measured by the oral bacteria quantification system, could be estimated by using ATP bioluminescence assay. It was suggested that this assay could be used for quantitative evaluation of the effect of oral cleaning.

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来源期刊

Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.