Mehmet Tolga Kırış, Sefa Ergün, Ozan Akıncı, Sevgi Ergin, Mehmet Velidedeoğlu, Bekir Sami Kocazeybek, Ertuğrul Göksoy
{"title":"聚合酶链反应(PCR)对肝囊性包虫病棘球蚴DNA的评价。","authors":"Mehmet Tolga Kırış, Sefa Ergün, Ozan Akıncı, Sevgi Ergin, Mehmet Velidedeoğlu, Bekir Sami Kocazeybek, Ertuğrul Göksoy","doi":"10.47717/turkjsurg.2022.5427","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this study was to determine the DNA and genotypes of <i>Echinococcus granulosus</i> in liver cyst hydatids isolated in humans.</p><p><strong>Material and methods: </strong>This study was conducted prospectively at the Department of General Surgery of the Cerrahpaşa School of Medicine, University of İstanbul-Cerrahpaşa, between January 2015 and June 2016 in 30 patients who were operated on for cystic <i>Echinococcosis. E. granulosus</i> DNA was analyzed using the Polymerase Chain Reaction (PCR) method in the cyst samples (protoscolex and/or germinative membrane) obtained during the operation, and genotype was determined in the PCR positive samples by sequence analysis. At the same time, indirect hemagglutination (IHA) was used to test for the presence of antibodies in the patients' blood.</p><p><strong>Results: </strong><i>E. granulosus</i> DNA was found in 29 out of 30 cystic <i>Echinococcosis</i> of the liver samples. All of the 29 cystic <i>Echinococcosis</i> samples were found to be G1 (sheep) species. Also, IHA was positive in 22 patients and negative in eight patients.</p><p><strong>Conclusion: </strong>In the present study, G1 species was the most commonly seen liver cystic <i>Echinococcosis</i> species. We suggest that a vaccine, which could be developed against prevalent regional genotypes, would be efficacious in the prevention of the disease with a cause of mortality and morbidity.</p>","PeriodicalId":23374,"journal":{"name":"Turkish Journal of Surgery","volume":"38 2","pages":"196-201"},"PeriodicalIF":0.5000,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714648/pdf/TJS-38-196.pdf","citationCount":"0","resultStr":"{\"title\":\"Evaluation of <em>Echinococcus</em> DNA by polymerase chain reaction (PCR) in cystic <em>Echinococcosis</em> of the liver.\",\"authors\":\"Mehmet Tolga Kırış, Sefa Ergün, Ozan Akıncı, Sevgi Ergin, Mehmet Velidedeoğlu, Bekir Sami Kocazeybek, Ertuğrul Göksoy\",\"doi\":\"10.47717/turkjsurg.2022.5427\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>The aim of this study was to determine the DNA and genotypes of <i>Echinococcus granulosus</i> in liver cyst hydatids isolated in humans.</p><p><strong>Material and methods: </strong>This study was conducted prospectively at the Department of General Surgery of the Cerrahpaşa School of Medicine, University of İstanbul-Cerrahpaşa, between January 2015 and June 2016 in 30 patients who were operated on for cystic <i>Echinococcosis. E. granulosus</i> DNA was analyzed using the Polymerase Chain Reaction (PCR) method in the cyst samples (protoscolex and/or germinative membrane) obtained during the operation, and genotype was determined in the PCR positive samples by sequence analysis. At the same time, indirect hemagglutination (IHA) was used to test for the presence of antibodies in the patients' blood.</p><p><strong>Results: </strong><i>E. granulosus</i> DNA was found in 29 out of 30 cystic <i>Echinococcosis</i> of the liver samples. All of the 29 cystic <i>Echinococcosis</i> samples were found to be G1 (sheep) species. Also, IHA was positive in 22 patients and negative in eight patients.</p><p><strong>Conclusion: </strong>In the present study, G1 species was the most commonly seen liver cystic <i>Echinococcosis</i> species. We suggest that a vaccine, which could be developed against prevalent regional genotypes, would be efficacious in the prevention of the disease with a cause of mortality and morbidity.</p>\",\"PeriodicalId\":23374,\"journal\":{\"name\":\"Turkish Journal of Surgery\",\"volume\":\"38 2\",\"pages\":\"196-201\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2022-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714648/pdf/TJS-38-196.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Turkish Journal of Surgery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47717/turkjsurg.2022.5427\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"SURGERY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish Journal of Surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47717/turkjsurg.2022.5427","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"SURGERY","Score":null,"Total":0}
Evaluation of Echinococcus DNA by polymerase chain reaction (PCR) in cystic Echinococcosis of the liver.
Objectives: The aim of this study was to determine the DNA and genotypes of Echinococcus granulosus in liver cyst hydatids isolated in humans.
Material and methods: This study was conducted prospectively at the Department of General Surgery of the Cerrahpaşa School of Medicine, University of İstanbul-Cerrahpaşa, between January 2015 and June 2016 in 30 patients who were operated on for cystic Echinococcosis. E. granulosus DNA was analyzed using the Polymerase Chain Reaction (PCR) method in the cyst samples (protoscolex and/or germinative membrane) obtained during the operation, and genotype was determined in the PCR positive samples by sequence analysis. At the same time, indirect hemagglutination (IHA) was used to test for the presence of antibodies in the patients' blood.
Results: E. granulosus DNA was found in 29 out of 30 cystic Echinococcosis of the liver samples. All of the 29 cystic Echinococcosis samples were found to be G1 (sheep) species. Also, IHA was positive in 22 patients and negative in eight patients.
Conclusion: In the present study, G1 species was the most commonly seen liver cystic Echinococcosis species. We suggest that a vaccine, which could be developed against prevalent regional genotypes, would be efficacious in the prevention of the disease with a cause of mortality and morbidity.
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