外泌体介导的lncRNA LINC01140通过调节Wnt/β-Catenin通路减缓乳腺癌进展

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zhe Guo, Yang Li, Wenhuan Li, Hai Li, Zhiyong Wu
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引用次数: 0

摘要

外泌体递送的长链非编码rna在癌症控制中起作用。目前尚不清楚外泌体LINC01140如何促进乳腺癌(BC)的生长。本研究的目的是确定外泌体LINC01140在乳腺癌发展中的功能。采用定量逆转录聚合酶链反应检测LINC01140的表达。为了研究LINC01140过表达对BC细胞增殖的影响,采用了CCK-8和集落形成试验(CFA)。Western blotting检测凋亡相关蛋白(Bax、Bcl-2)和Wnt/β-catenin信号通路相关蛋白(Wnt、C-myc、β-catenin、p-GSK-3β)的表达。通过western blotting检测BC细胞外泌体的CD63和CD9水平。为了研究外泌体LINC01140对裸鼠Wnt/β-catenin信号通路和异种移植肿瘤的影响,我们获得了过表达LINC01140的BC细胞外泌体,并与BC细胞共培养。在BC中,发现LINC01140表达不良。过表达LINC01140抑制BC细胞增殖,降低Bcl-2、β-catenin、C-myc、Wnt蛋白水平,升高Bax、p-GSK-3蛋白水平。此外,外泌体LINC01140阻碍了Wnt/β-catenin信号通路的激活,导致体内乳腺癌细胞生长下降。外泌体LINC01140的存在阻碍了Wnt/β-catenin的启动,并降低了BC细胞的癌性特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exosome-Mediated lncRNA LINC01140 Attenuates Breast Cancer Progression by Regulating the Wnt/β-Catenin Pathway.

Exosome-delivered long non-coding RNAs have a role in the cancer control. It is unknown how exosomal LINC01140 contributes to the breast cancer (BC) growth. The purpose of this investigation is to identify exosomal LINC01140's function in the development of breast cancer. Using quantitative reverse transcripion polymerase chain reaction, the expression of LINC01140 was measured. To investigate how LINC01140 overexpression impacts BC cell proliferation, CCK-8 as well as colony formation assays (CFA) were employed. The expression of apoptosis-related proteins (Bax and Bcl-2) and Wnt/β-catenin signal pathway-related proteins (Wnt, C-myc, β-catenin, and p-GSK-3β) was assessed through Western blotting. Exosomes from BC cells were verified by western blotting to measure CD63 and CD9 levels. To examine how exosomal LINC01140 affects Wnt/β-catenin signaling pathway and xenograft tumor in nude mice, BC cell exosomes that were overexpressing LINC01140 were obtained and co-cultured with BC cells. In BC, it was discovered that LINC01140 had poor expression. BC cell proliferation was inhibited by overexpressing LINC01140, and the levels of the proteins Bcl-2, β-catenin, C-myc, and Wnt were lowered while Bax and p-GSK-3 were increased. In addition, exosomal LINC01140 hindered the activation of the Wnt/β-catenin signaling pathway, leading to a decrease in the growth of breast cancer cells in vivo. The presence of exosomal LINC01140 impedes the initiation of Wnt/β-catenin and reduces the cancerous characteristics of BC cells.

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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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