鼠腹股沟部位心脏移植模型--研究外周淋巴结在移植中作用的适用工具。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
ACS Applied Electronic Materials Pub Date : 2024-03-01 Epub Date: 2023-08-07 DOI:10.1111/xen.12817
Yaguang Li, Wenjia Yuan, Mingda Zhong, Julia Qi, Xinguo Zheng, Xubiao Xie, Tengfang Li, Hedong Zhang, Xin Jiang, Longkai Peng, Helong Dai
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引用次数: 0

摘要

小鼠异位心脏移植模型已被广泛用于研究抗原特异性免疫反应或新的免疫抑制剂,这与外周淋巴结有很大关系。因此,需要一种适用于相关研究的新器官移植模型。在这里,我们描述了一种腹股沟部位小鼠心脏移植模型,该模型采用袖带技术,将供体主动脉和肺动脉吻合到受体的截断股血管上。BALB/c-C57BL/6异体移植组移植物的平均存活时间(MST)为7.2±0.3天,BALB/c-Sprague-Dawley(SD)大鼠异体移植组移植物的平均存活时间(MST)为1.9±0.2天。H&E 结果显示,两组供体心脏在终点时均表现出典型的病理特征。埃文斯蓝描记显示,移植侧后肢的腘窝淋巴结比对侧大。此外,CD3、CD20 的 IHC 染色显示,移植侧腘淋巴结的生发中心和皮质区明显比对侧增多。总之,该模型可作为研究外周淋巴结在器官移植排斥反应中作用的理想模型。此外,腹膜外移植在 3R 原则(Replacement、Reduction、Refinement)下的动物福利方面也向前迈进了一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A murine groin site cardiac transplantation model-applicable tool for studying roles of peripheral lymph nodes in transplantation.

The murine heterotopic cardiac transplantation model has been widely used to study antigen-specific immune responses or new immunosuppressive agents, which have a strong correlation with peripheral lymph nodes. Thus, a new organ transplantation model that is applicable to related studies is needed. Here, we describe a groin-site murine heart transplantation model using a cuff technique, in which the donor aorta and pulmonary artery are anastomosed to the truncated femoral vessels of the recipient. The mean survival time (MST) of the grafts in BALB/c-to-C57BL/6 allo-transplant group was 7.2 ± 0.3 days, and 1.9 ± 0.2 days in BALB/c-to-Sprague-Dawley (SD) rat xeno-transplant group. H&E results show that donor hearts from both groups demonstrate typical pathological features at the endpoint. Evans Blue tracing revealed that the popliteal lymph nodes of the grafted side hindlimb are larger than those of the contralateral side. Moreover, IHC staining for CD3, CD20 shows that the germinal center and cortex region of the grafted side of popliteal lymph nodes is apparently increased than that of the contralateral side. To sum up, this model may serve as an ideal model to study the role of peripheral lymph nodes in organ transplant rejection. In addition, extra-peritoneal grafting makes a step forward in animal welfare under the 3Rs' principle (Replacement, Reduction, Refinement).

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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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