大麻酚2型受体激活在大鼠单侧输尿管梗阻诱导的肾纤维化中的作用。

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mahmoud El Tohamy, Mohamed Adel, Fayza Rashad El-Menabawy, Gad El Mawla Gad, Randa El-Gamal, Hanaa El Serougy
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引用次数: 0

摘要

背景:慢性肾脏疾病(CKD)主要以肾纤维化结束。CB2受体对肾纤维化的作用尚不清楚。本研究旨在探讨CB2受体对肾纤维化的影响及其机制。方法:将50只成年雄性Sprague-Dawley大鼠分为5组;正常,假手术;只对大鼠的输尿管进行UUO操作;大鼠左输尿管结扎,JWH后;结扎大鼠的左输尿管,并在JWH前+后接受JWH 133治疗14天;大鼠在UUO手术前后接受JWH133治疗14天。血清肌酐和BUN与组织MDA、GSH和过氧化氢酶一起进行评估。用H&E和Masson三色染色法对肾组织进行组织病理学评价。对TGF-β1、AQP1、Caspase-3、LC3B和p62进行免疫组织化学染色。定量RT-PCR检测AQP1和CB2受体基因的表达。结果:UUO引起肾组织严重损伤,肾功能参数降低,胶原沉积增加,TGF-β1增加,AQP1表达降低。结论:JWH-133对这些参数的改善表明CB2受体激活通过减少氧化应激、细胞凋亡和自噬发挥抗纤维化作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of Cannabinoid Type 2 Receptor Activation in Renal Fibrosis Induced by Unilateral Ureteric Obstruction in Rats.

Background: Chronic kidney disease (CKD) ends mostly with renal fibrosis. The effect of CB2 receptor on renal fibrosis has been unclear. The aim of this study was to investigate the effect of CB2 receptor on renal fibrosis and the mechanisms behind it.

Methods: 50 adult male Sprague-Dawley rats were divided into 5 groups; normal, sham; rats had their ureters only manipulated, UUO; rats had their left ureters ligated, and JWH post; rats had their left ureters ligated and they received JWH 133 for 14 days, JWH pre+post; rats received JWH 133 for 14 days before and after UUO procedure. Serum creatinine and BUN were assessed together with tissue MDA, GSH, and catalase. Histopathological evaluation of the renal tissue by H&E and Masson's trichrome was done. Immunohistochemical staining for TGF-β1, AQP1, Caspase-3, LC3B and p62 was performed. AQP1 and CB2 receptors genes expression was detected by quantitative RT-PCR.

Results: UUO had caused severe damage in the renal tissue with reduction of the renal function parameter accompanied by increase in the collagen deposition with increase TGF-β1 and decrease AQP1 expression.

Conclusions: The improvement of these parameters with JWH-133 suggests an anti-fibrotic role of CB2 receptor activation through reduction of oxidative stress, apoptosis, and autophagy.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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