AIE能够对自噬水平不同的活细胞亚群进行无转染鉴定和分离。

IF 14.6 1区生物学 Q1 CELL BIOLOGY

Autophagy Pub Date : 2023-12-01 Epub Date: 2023-08-02 DOI:10.1080/15548627.2023.2235197

Wenbin Zhang, Pengfei Wei, Liu Liu, Tao Ding, Yinyin Yang, Peipei Jin, Li Zhang, Zhibin Zhao, Meimei Wang, Bochuan Hu, Xin Jin, Zeng Xu, Han Zhang, Yang Song, Liansheng Wang, Suqin Zhong, Jing Chen, Zhenyu Yang, Ziying Chen, Yu Wu, Zhiming Ye, Youcui Xu, Yunjiao Zhang, Long-Ping Wen

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引用次数: 0

摘要

缩写：3-MA，3-甲基腺嘌呤；AIE，聚集诱导发射；AIEgens，聚集诱导发射发光原；ATG5，自噬相关5；骨髓源性巨噬细胞；CQ、氯喹；DiD，1,1'-二十八烷基-3,3,3'-四甲基吲哚二碳花青高氯酸盐；高氯酸二O，3,3'-二十八碳酰氧羰基花青；DMSO、二甲基亚砜；d-THP-1、分化的THP-1；FACS，荧光激活细胞分选；FBS，胎牛血清；FCCP，羰基氰化物4-（三氟甲氧基）苯基腙；GABARAP，GABA型受体相关蛋白；绿色荧光蛋白；HBSS，汉克斯平衡盐溶液；高效液相色谱法；HRP、辣根过氧化物酶；IL1B，白细胞介素1β；KT，由细胞穿透肽和AIEgen四苯基乙烯组成的AIE探针；脂化LC3；LDH、乳酸脱氢酶；LIR、LC3相互作用区；LKR，由LC3相互作用肽、细胞穿透肽和非AIE荧光分子罗丹明组成的工程分子探针；LKT，由LC3相互作用肽、细胞穿透肽和AIEgen四苯基乙烯组成的工程分子探针；LPS、脂多糖；MAP1LC3/LC3、微管相关蛋白1轻链3；小鼠胚胎成纤维细胞；mRFP，单体红色荧光蛋白；NHS，N-羟基琥珀酰亚胺；NLRP3，含有3个的NLR家族pyrin结构域；PBS、磷酸盐缓冲盐水；PCC，pearson相关系数；PL、光致发光；PMA、佛波醇12-肉豆蔻酸酯13-乙酸酯；RAP、雷帕霉素；RIM，分子内运动的限制；s.e.m，平均值的标准误差；SPR，表面等离子体共振；SQSTM1/p62，螯合体1；TAX1BP1、Tax1结合蛋白1；四苯基乙烯；TPE炔，1-（4-乙炔基苯基）-1,2,2-三苯基乙烯；Tre，海藻糖；u-THP-1：未分化的THP-1；紫外线可见，紫外线可见。

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AIE-enabled transfection-free identification and isolation of viable cell subpopulations differing in the level of autophagy.

Abbreviations: 3-MA, 3-methyladenine; AIE, aggregation-induced emission; AIEgens, aggregation-induced emission luminogens; ATG5, autophagy related 5; BMDM, bone marrow-derived macrophage; CQ, chloroquine; DiD, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate; DiO, 3,3'-dioctadecyloxacarbocyanine perchlorate; DMSO, dimethyl sulfoxide; d-THP-1, differentiated THP-1; FACS, fluorescence activated cell sorting; FBS, fetal bovine serum; FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; GABARAP, GABA type A receptor-associated protein; GFP, green fluorescent protein; HBSS, Hanks' balanced salt solution; HPLC, high-performance liquid chromatography; HRP, horseradish peroxidase; IL1B, interleukin 1 beta; KT, an AIE probe composed of a cell-penetrating peptide and an AIEgen tetraphenyl ethylene; LC3-II, lipidated LC3; LDH, lactate dehydrogenase; LIR, LC3-interacting region; LKR, engineered molecular probe composed of an LC3-interacting peptide, a cell-penetrating peptide and a non-AIE fluorescent molecule rhodamine; LKT, engineered molecular probe composed of an LC3-interacting peptide, a cell-penetrating peptide and an AIEgen tetraphenyl ethylene; LPS, lipopolysaccharide; MAP1LC3/LC3, microtubule associated protein 1 light chain 3; MEF, mouse embryonic fibroblast; mRFP, monomeric red fluorescent protein; NHS, N-hydroxysuccinimide; NLRP3, NLR family pyrin domain containing 3; PBS, phosphate-buffered saline; PCC, pearson's correlation coefficient; PL, photoluminescence; PMA, phorbol 12-myristate 13-acetate; RAP, rapamycin; RIM, restriction of intramolecular motions; s.e.m., standard error of the mean; SPR, surface plasmon resonance; SQSTM1/p62, sequestosome 1; TAX1BP1, Tax1 binding protein 1; TPE, tetraphenylethylene; TPE-yne, 1-(4-ethynylphenyl)-1,2,2-triphenylethene; Tre, trehalose; u-THP-1: undifferentiated THP-1; UV-Vis, ultraviolet visible.

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来源期刊

Autophagy 生物-细胞生物学

CiteScore

21.30

自引率

2.30%

发文量

277

审稿时长

1 months

期刊介绍： Autophagy is a peer-reviewed journal that publishes research on autophagic processes, including the lysosome/vacuole dependent degradation of intracellular material. It aims to be the premier journal in the field and covers various connections between autophagy and human health and disease, such as cancer, neurodegeneration, aging, diabetes, myopathies, and heart disease. Autophagy is interested in all experimental systems, from yeast to human. Suggestions for specialized topics are welcome. The journal accepts the following types of articles: Original research, Reviews, Technical papers, Brief Reports, Addenda, Letters to the Editor, Commentaries and Views, and Articles on science and art. Autophagy is abstracted/indexed in Adis International Ltd (Reactions Weekly), EBSCOhost (Biological Abstracts), Elsevier BV (EMBASE and Scopus), PubMed, Biological Abstracts, Science Citation Index Expanded, Web of Science, and MEDLINE.