以蜜环菌为例进行担子菌门基因组组装和注释的实用方法。

IF 2.2 4区 工程技术 Q3 BIOCHEMICAL RESEARCH METHODS
BioTechniques Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI:10.2144/btn-2023-0023
Deborah L Narh Mensah, Brenda D Wingfield, Martin Pa Coetzee
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引用次数: 0

摘要

基因组测序、组装和注释平台以及算法的技术进步导致了几项基因组研究,这为我们进一步了解包括蜜环菌在内的植物病原体的生物学创造了机会。大多数蜜环菌属物种都是兼性坏死营养菌,通常在木本植物上引起根和茎腐烂,对世界各地的农业和林业产生重大影响。本文就蜜环菌基因组项目中使用的样品和应用的方法对基因组测序、组装和注释进行了评价。制定了信息图指南和资源数据库,以促进未来的蜜环菌基因组项目。综述了从蜜环菌基因组研究中获得的知识,并对进一步研究提供了展望。本指南可应用于其他二倍体和双核真菌基因组学。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A practical approach to genome assembly and annotation of Basidiomycota using the example of Armillaria.

Technological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome projects are evaluated in this review. Infographic guidelines and a database of resources to facilitate future Armillaria genome projects were developed. Knowledge gained from genomic studies of Armillaria species is summarized and prospects for further research are provided. This guide can be applied to other diploid and dikaryotic fungal genomics.

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来源期刊
BioTechniques
BioTechniques 工程技术-生化研究方法
CiteScore
4.40
自引率
0.00%
发文量
68
审稿时长
3.3 months
期刊介绍: BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.
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