干细胞在三维支架中的培养和分化。

3区生物学 Q2 Biochemistry, Genetics and Molecular Biology

Progress in molecular biology and translational science Pub Date : 2023-01-01 Epub Date: 2023-07-26 DOI:10.1016/bs.pmbts.2023.04.009

Yasuhiko Tabata, Idaszek Joanna, Akon Higuchi

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引用次数: 0

摘要

常规的二维（2-D）培养在标准技术中易于用于人多能干（hPS）细胞培养，并且对于分析或开发信号通路以保持在2-D细胞培养材料上培养的hPS细胞的多能干状态是重要的。然而，制备hPS细胞的最有效方案是在三维（3-D）培养单元中进行细胞培养，因为在临床治疗中应该使用大量的hPS细胞。考虑了hPS细胞的一些三维培养策略：（a）在悬浮水凝胶中进行微胶囊化细胞培养，（b）在微载体（MC）上进行细胞培育，（c）在自聚集球体[细胞聚集体；胚胎体（EB）和类器官]上进行细胞培养，（D）在微纤维或纳米纤维上进行细胞培养，以及（e）在大孔支架中进行细胞培养。本章介绍了这些栽培方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Stem cell culture and differentiation in 3-D scaffolds.

Conventional two-dimensional (2-D) cultivation are easy to utilize for human pluripotent stem (hPS) cell cultivation in standard techniques and are important for analysis or development of the signal pathways to keep pluripotent state of hPS cells cultivated on 2-D cell culture materials. However, the most efficient protocol to prepare hPS cells is the cell culture in a three dimensional (3-D) cultivation unit because huge numbers of hPS cells should be utilized in clinical treatment. Some 3-D cultivation strategies for hPS cells are considered: (a) microencapsulated cell cultivation in suspended hydrogels, (b) cell cultivation on microcarriers (MCs), (c) cell cultivation on self-aggregated spheroid [cell aggregates; embryoid bodies (EBs) and organoids], (d) cell cultivation on microfibers or nanofibers, and (e) cell cultivation in macroporous scaffolds. These cultivation ways are described in this chapter.

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来源期刊

Progress in molecular biology and translational science 生物-生化与分子生物学

CiteScore

6.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Progress in Molecular Biology and Translational Science (PMBTS) provides in-depth reviews on topics of exceptional scientiﬁc importance. If today you read an Article or Letter in　Nature　or a Research Article or Report in　Science　reporting ﬁndings of exceptional importance, you likely will ﬁnd comprehensive coverage of that research area in a future　PMBTS　volume.