从耕地地下土壤中分离出一种产生新型放线菌地链霉菌的抗菌硫肽。

Stanzin Choksket, Mahaldeep Kaur, Anil Kumar Pinnaka, Suresh Korpole
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引用次数: 0

摘要

从土壤中分离到一株革兰氏阳性、需氧、非运动和丝状放线菌SKN60T,经16S rRNA基因序列分析,该菌株与laurentii链霉菌ATCC 31255T和S. roseicoloratus TRM 44457T的同源性分别为99.3%和99.0%。结果表明,玫瑰色紫荆TRM 44457T的ANI和AAI最高,分别为88.45%和85.61%。与S. roseicoloratus TRM 44457T同源性为33.7%。在系统基因组树中与S. roseicoloratus TRM 44457T和S. laurentii ATCC 31255T形成集群。细胞壁分析显示,二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱是主要的极性脂质,二氨基苯甲酸是诊断的二氨基酸。主要脂肪酸为iso-C15:0、前iso-C15:0和iso-C16:0。G+C含量为72.3 mol%。利用anti - smash数据库进行基因组序列分析,发现一个硫肽生物合成基因簇与伯纳霉素的相似性为94%。1146da的质量与贝尼霉素相同。但硫肽和贝尼霉素的前导肽序列存在细微差异。值得注意的是,没有有效的报道,因此SKN60T是唯一含有贝纳霉素BGC的菌株,因为没有其他系统发育亲缘关系。此外,菌株SKN60T在表型和遗传特征上与链霉菌属的所有亲缘系存在差异。因此,建议将其作为新种命名为Streptomyces terrae sp. nov.菌株SKN60T (=MTCC 13163T;= JCM 35768t)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An antimicrobial thiopeptide producing novel actinomycetes <i>Streptomyces terrae</i> sp. nov., isolated from subsurface soil of arable land.

An antimicrobial thiopeptide producing novel actinomycetes <i>Streptomyces terrae</i> sp. nov., isolated from subsurface soil of arable land.

An antimicrobial thiopeptide producing novel actinomycetes <i>Streptomyces terrae</i> sp. nov., isolated from subsurface soil of arable land.

An antimicrobial thiopeptide producing novel actinomycetes Streptomyces terrae sp. nov., isolated from subsurface soil of arable land.

An antimicrobial producing Gram-positive, aerobic, nonmotile, and filamentous actinobacterial strain SKN60T was isolated from soil The isolate exhibited 99.3% and 99.0% identity with Streptomyces laurentii ATCC 31255T and S. roseicoloratus TRM 44457T, respectively, in 16S rRNA gene sequence analysis. However, the genome sequence displayed maximum ANI (88.45%) and AAI (85.61%) with S. roseicoloratus TRM 44457T. Similarly, the dDDH showed 33.7% identity with S. roseicoloratus TRM 44457T. It formed a cluster with S. roseicoloratus TRM 44457T and S. laurentii ATCC 31255T in phylogenomic tree. Cell wall analysis revealed the presence of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine as major polar lipids and diaminopimelic acid as diagnostic diamino acid. Major fatty acids were iso-C15:0, anteiso-C15:0, and iso-C16:0. The G+C content was found to be 72.3 mol%. Genome sequence analysis using antiSMASH database showed occurrence of a thiopeptide biosynthesis gene cluster with 94% similarity to berninamycin from S. bernensis UC5144. The mass of 1146 Da is identical with berninamycin. But subtle differences observed in leader peptide sequence of thiopeptide and berninamycin. Notably, S. bernensis is not validly reported and thus SKN60T is the only strain containing berninamycin BGC as no other phylogenetic relative had it. Additionally, strain SKN60T differed in phenotypic and genetic characteristics with all phylogenetic relatives of the genus Streptomyces. Therefore, it is proposed as a novel species with the name Streptomyces terrae sp. nov. strain SKN60T (=MTCC 13163T; = JCM 35768T).

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CiteScore
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