硒纳米粒子和枣肉提取物对四氯化碳诱导的白化大鼠肝损伤的保肝作用比较研究。

IF 1.4 Q3 ANATOMY & MORPHOLOGY
Anatomy & Cell Biology Pub Date : 2023-12-31 Epub Date: 2023-09-12 DOI:10.5115/acb.23.101
Ghada Nady Ouais, Doaa Mohamad Hassan
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引用次数: 0

摘要

暴露于四氯化碳(CCL4)等环境污染物会导致肝损伤。本研究旨在比较枣肉提取物(DFE)和硒纳米颗粒(SeNPs)对 CCL4 引起的肝毒性的改善活性,以及 DFE 是否可以作为一种有用的替代补充剂。研究人员招募了 24 只雄性白化大鼠,并将其随机分为 4 组(每组 6 只)。第二组大鼠腹腔注射 CCL4,剂量为 0.5 毫克/千克,每周两次,连续四周。第三组大鼠接受 SeNPs 预处理,剂量为 2.5 毫克/千克,每天一次,每周三次,单独使用四周,然后与之前所述剂量的 CCL4 联合使用四周。第 IV 组大鼠先以 8 毫升水提取物/千克/天的剂量口服 DFE,单独治疗四周,然后再与之前所述剂量的 CCL4 联合治疗四周。评估肝损伤的方法是估计血浆中的白蛋白浓度、丙氨酸氨基转移酶活性和组织基因表达。通过测量丙二醛、超氧化物歧化酶和总谷胱甘肽的组织浓度来评估肝脏氧化水平。此外,还对炎症介质肿瘤坏死因子--α和白细胞介素-6进行了估计。通过组织病理学和免疫组化检查来检测肝脏细胞结构的损伤。这项研究表明,无论是使用昂贵的 SeNPs 还是经济的 DFE,都能保护大鼠因 CCL4 引起的肝损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A comparative study on the hepatoprotective effect of selenium-nanoparticles and dates flesh extract on carbon tetrachloride induced liver damage in albino rats.

Exposure to environmental pollutants such as carbon tetrachloride (CCL4) causes liver damage. This study aimed to compare the ameliorative activity of the dates flesh extract (DFE) and selenium-nanoparticles (SeNPs) on CCL4-induced hepatotoxicity and if DFE could be a useful alternative supplement. Twenty-four male albino rats were enrolled and randomly divided into four equal groups (6 rats in each group): control group received only basal diet with no medications. Group II received CCL4 in a dose of 0.5 mg/kg intraperitoneal injection twice weekly for four weeks. Group III rats were pretreated with SeNPs in a dose of 2.5 mg/kg once a day orally three times/wk for four weeks alone then combined with the previously described dose of CCL4 for another four weeks. Group IV rats were pretreated with DFE in a dose of 8 ml of the aqueous extract/kg/d orally for four weeks alone then combined with the previously described dose of CCL4 for another four weeks. The liver damage was assessed by estimation of plasma concentration of albumin and enzymes activities of alanine aminotransferase and tissue genes expression. Liver oxidation levels were assessed by measuring the tissue concentration of the malondialdehyde, superoxide dismutase, and the total glutathione. Additionally, inflammatory mediators tumour necrosis factor--α and interleukin-6 were estimated. Detecting the liver's cellular structural damage was done by histopathological and immunohistochemical examination. This study suggests that CCL4-induced liver damage in rats can be protected by administration whether the costly SeNPs or the economical DFE.

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来源期刊
Anatomy & Cell Biology
Anatomy & Cell Biology ANATOMY & MORPHOLOGY-
CiteScore
1.80
自引率
9.10%
发文量
75
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