一种基于细胞杂交的产生用于检测细胞因子的重组兔单克隆抗体的方法。

IF 2.2 4区工程技术 Q3 BIOCHEMICAL RESEARCH METHODS

BioTechniques Pub Date : 2023-10-01 Epub Date: 2023-09-06 DOI:10.2144/btn-2023-0026

Yu Peng, Chun'e Zhang, Minyan Deng, Haijuan Jiang, Huishu Huang, Yue Li, Weiping Lai, Yu-Pin Lin, Jun Yu

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引用次数: 0

摘要

重组兔单克隆抗体（rabbitrAbs）已在各种生物医学领域显示出前景。然而，使用传统技术产生兔rAb具有挑战性且成本高昂。在这里，我们描述一种方便且具有成本效益的方法。使用这种方法，我们产生了针对小鼠可溶性IL-6受体α的兔rAbs，其亲和力在10-9至10-12M范围内。所提出的方法适用于希望为研究定制兔rAb的工业和学术科学家。

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A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines.

Recombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor α with affinities in the range of 10^-9 to 10^-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research.

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来源期刊

BioTechniques 工程技术-生化研究方法

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

3.3 months

期刊介绍： BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.