一个新的lncRNA FUAT1/TNS4轴通过抑制活性氧介导的胃癌细胞凋亡而赋予化疗耐药。

IF 5.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Antioxidants & redox signaling Pub Date : 2024-07-01 Epub Date: 2023-11-15 DOI:10.1089/ars.2023.0298
Mingliang Liu, Hehe Li, Xiaoling Li, Boyu Pan, Jian Zhang, Ya Pan, Miaomiao Shen, Liren Liu
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引用次数: 0

摘要

目的:活性氧(Reactive oxygen species, ROS)在大多数化疗药物的细胞毒性和耐药性传递中起着至关重要的作用。因此,全面了解癌细胞中抗氧化应激的复杂活动可能为发现化学耐药的共同机制提供有价值的见解。结果:我们通过基于转录组测序(RNA- seq)技术的独特筛选策略,鉴定了一种新的长链非编码RNA (lncRNA),称为氟尿嘧啶相关转录-1 (FUAT1),作为ros介导的内在化学耐药的关键非遗传参与者。为了研究FUAT1调控轴在化疗耐药中的确切作用,我们进行了一系列体外和体内实验,包括功能增益/丧失和拯救实验。在机制上,我们的研究结果表明FUAT1通过海绵miR-140-5p上调Tensin 4 (TNS4),从而通过抑制ros介导的细胞凋亡使胃癌细胞在化疗中存活。临床研究发现,在5-氟尿嘧啶辅助化疗的胃癌和结肠癌患者中,FUAT1/TNS4调节轴与总生存期和无进展生存期呈负相关。创新:我们设计了一种新的筛选策略,不同于传统的使用耐药菌株的方法。通过这种方法,我们发现了之前未被识别的lncRNA FUAT1/TNS4轴,该轴在ros介导的内在化学耐药中起关键作用。结论:我们的研究结果揭示了癌细胞对化疗反应的基本适应机制,并为开发旨在克服化疗耐药的策略提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Novel lncRNA FUAT1/TNS4 Axis Confers Chemoresistance by Suppressing Reactive Oxygen Species-Mediated Apoptosis in Gastric Cancer.
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来源期刊
Antioxidants & redox signaling
Antioxidants & redox signaling 生物-内分泌学与代谢
CiteScore
14.10
自引率
1.50%
发文量
170
审稿时长
3-6 weeks
期刊介绍: Antioxidants & Redox Signaling (ARS) is the leading peer-reviewed journal dedicated to understanding the vital impact of oxygen and oxidation-reduction (redox) processes on human health and disease. The Journal explores key issues in genetic, pharmaceutical, and nutritional redox-based therapeutics. Cutting-edge research focuses on structural biology, stem cells, regenerative medicine, epigenetics, imaging, clinical outcomes, and preventive and therapeutic nutrition, among other areas. ARS has expanded to create two unique foci within one journal: ARS Discoveries and ARS Therapeutics. ARS Discoveries (24 issues) publishes the highest-caliber breakthroughs in basic and applied research. ARS Therapeutics (12 issues) is the first publication of its kind that will help enhance the entire field of redox biology by showcasing the potential of redox sciences to change health outcomes. ARS coverage includes: -ROS/RNS as messengers -Gaseous signal transducers -Hypoxia and tissue oxygenation -microRNA -Prokaryotic systems -Lessons from plant biology
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