建立基于VP1蛋白的间接ELISA法检测猪sapelvirus IgG抗体。

IF 1.1 4区 农林科学 Q2 Veterinary
Jiajia Liu, Benqiang Li, Jie Tao, Jinghua Cheng, Ying Shi, Changtao Qiao, Xiaohui Shen, Huili Liu
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引用次数: 0

摘要

猪sapelovirus (PSV)是一种在中国广泛流行的新型肠道病毒。由于目前还没有针对PSV的临床血清学检测,本研究的目的是建立一种间接酶联免疫吸附试验(i-ELISA)来检测猪PSV免疫球蛋白G (IgG)抗体。一种名为SHPD202148的PSV毒株首次从仔猪粪便样本中分离出来。其结构蛋白VP1在pET表达系统中进行原核表达,并进行纯化。以具有反应原性的重组蛋白为包被抗原,检测限为1:12 800稀释,检测截止值为0.352,灵敏度和特异性高。最后,采用血清中和(SN)试验对不同猪群的现场血清进行平行检测。结果阳性126份,阴性36份,两者的符合率为97.0%。该酶联免疫吸附试验可作为检测血清中抗PSV抗体的血清学检测方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of an indirect ELISA method based on the VP1 protein for detection of IgG antibodies against porcine sapelovirus.

Porcine sapelovirus (PSV) is a newly emerging enterovirus that is widely prevalent in China. Since there is no clinical serological testing for PSV, the objective of this study was to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) for detection of PSV immunoglobulin G (IgG) antibody in pigs. A PSV strain, named SHPD202148, was first isolated from the fecal samples of piglets. Its structural protein, VP1, was prokaryotic-expressed in the pET expression system, followed by purification. Using the recombinant protein with reactogenicity as coating antigen, an i-ELISA, characterized by high sensitivity and specificity, had a detection limit at 1:12 800 dilution with a determined cutoff value of 0.352. Finally, field sera collected from different pig herds were tested in parallel by the serum neutralization (SN) test. The result showed that 126 samples were positive and 36 were negative, with an agreement of 97.0% in both cases. This i-ELISA can be used as an alternative serological test for detecting antibodies against PSV in blood serum.

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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
58
审稿时长
>24 weeks
期刊介绍: The Canadian Journal of Veterinary Research, published by the Canadian Veterinary Medical Association, is Canada''s only veterinary research publication. This quarterly peer-reviewed online-only journal has earned a wide international readership through the publishing of high quality scientific papers in the field of veterinary medicine. The Journal publishes the results of original research in veterinary and comparative medicine.
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