LGG-1/GGABARAP的膜定位对于秀丽隐杆线虫的自噬是可有可无的。

IF 14.6 1区生物学 Q1 CELL BIOLOGY

Autophagy Pub Date : 2023-12-01 Epub Date: 2023-08-23 DOI:10.1080/15548627.2023.2249393

Romane Leboutet, Céline Largeau, Emmanuel Culetto, Christophe Lefebvre, Thorsten Hoppe, Renaud Legouis

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引用次数: 0

摘要

LC3/GABARAP在大自噬/自噬中的大多数功能被认为取决于它们通过与脂质的结合与吞噬体膜的结合。通过定点诱变，我们抑制了秀丽隐杆线虫GABARAP的单一同源物LGG-1的结合。仅表达胞质形式的突变体揭示了切割形式的LGG-1在自噬中以及在自噬独立的胚胎功能中的重要作用。

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Membrane localization of LGG-1/GABARAP is dispensable for autophagy in C. elegans.

Most of the functions of LC3/GABARAP in macroautophagy/autophagy are considered to depend on their association with the phagophore membrane through a conjugation to a lipid. Using site-directed mutagenesis, we inhibited the conjugation of LGG-1, the single homolog of GABARAP in C. elegans. Mutants that express only cytosolic forms revealed an essential role for the cleaved form of LGG-1 in autophagy but also in an autophagy-independent embryonic function.

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来源期刊

Autophagy 生物-细胞生物学

CiteScore

21.30

自引率

2.30%

发文量

277

审稿时长

1 months

期刊介绍： Autophagy is a peer-reviewed journal that publishes research on autophagic processes, including the lysosome/vacuole dependent degradation of intracellular material. It aims to be the premier journal in the field and covers various connections between autophagy and human health and disease, such as cancer, neurodegeneration, aging, diabetes, myopathies, and heart disease. Autophagy is interested in all experimental systems, from yeast to human. Suggestions for specialized topics are welcome. The journal accepts the following types of articles: Original research, Reviews, Technical papers, Brief Reports, Addenda, Letters to the Editor, Commentaries and Views, and Articles on science and art. Autophagy is abstracted/indexed in Adis International Ltd (Reactions Weekly), EBSCOhost (Biological Abstracts), Elsevier BV (EMBASE and Scopus), PubMed, Biological Abstracts, Science Citation Index Expanded, Web of Science, and MEDLINE.