Carlos Ramiro Silva-Ramos, Juliana Noriega, Rafael F. Fajardo, Sandra M. Chala-Quintero, Adriana Del Pilar Pulido-Villamarín, Jairo Pérez-Torres, Rubiela Castañeda-Salazar, Claudia Cuervo
{"title":"哥伦比亚蝙蝠隐孢子虫分离株的分子检测和基因分型","authors":"Carlos Ramiro Silva-Ramos, Juliana Noriega, Rafael F. Fajardo, Sandra M. Chala-Quintero, Adriana Del Pilar Pulido-Villamarín, Jairo Pérez-Torres, Rubiela Castañeda-Salazar, Claudia Cuervo","doi":"10.1007/s11686-023-00697-8","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>Cryptosporidiosis is a zoonotic infectious disease caused by the protozoan parasite <i>Cryptosporidium</i> spp., frequently found in several animal species, including bats. Several <i>Cryptosporidium</i> genotypes have been described in bats worldwide, suggesting that bats are infected by host-specific <i>Cryptosporidium</i> spp. To date, there are no published reports about <i>Cryptosporidium</i> spp. in bats from Colombia. Therefore, this study aimed to determine the presence and molecular diversity of <i>Cryptosporidium</i> spp. in Colombian bats.</p><h3>Methods</h3><p>A total of 63 gut samples from three bat species served for molecular detection of <i>Cryptosporidium</i> spp. 18S rDNA gene by qPCR. The sequenced amplicons were used in subsequent phylogenetic analyses to identify them as species or genotypes.</p><h3>Results</h3><p><i>Cryptosporidium</i> spp. qPCR detection occurred in 9.5% (6/63) of bat intestines, and four sequences represented two new genotypes, called <i>Cryptosporidium</i> bat genotypes XIX and XX, were identified.</p><h3>Conclusions</h3><p>This study describes the detection of two novel <i>Cryptosporidium</i> bat genotypes, in two species of bats from a region of Colombia, requiring further studies to determine the relationhip between <i>Cryptosporidium</i> and bats in Colombia.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"68 3","pages":"676 - 682"},"PeriodicalIF":1.2000,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-023-00697-8.pdf","citationCount":"1","resultStr":"{\"title\":\"Molecular Detection and Genotyping of Cryptosporidium spp. Isolates from Bats in Colombia\",\"authors\":\"Carlos Ramiro Silva-Ramos, Juliana Noriega, Rafael F. Fajardo, Sandra M. Chala-Quintero, Adriana Del Pilar Pulido-Villamarín, Jairo Pérez-Torres, Rubiela Castañeda-Salazar, Claudia Cuervo\",\"doi\":\"10.1007/s11686-023-00697-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>Cryptosporidiosis is a zoonotic infectious disease caused by the protozoan parasite <i>Cryptosporidium</i> spp., frequently found in several animal species, including bats. Several <i>Cryptosporidium</i> genotypes have been described in bats worldwide, suggesting that bats are infected by host-specific <i>Cryptosporidium</i> spp. To date, there are no published reports about <i>Cryptosporidium</i> spp. in bats from Colombia. Therefore, this study aimed to determine the presence and molecular diversity of <i>Cryptosporidium</i> spp. in Colombian bats.</p><h3>Methods</h3><p>A total of 63 gut samples from three bat species served for molecular detection of <i>Cryptosporidium</i> spp. 18S rDNA gene by qPCR. The sequenced amplicons were used in subsequent phylogenetic analyses to identify them as species or genotypes.</p><h3>Results</h3><p><i>Cryptosporidium</i> spp. qPCR detection occurred in 9.5% (6/63) of bat intestines, and four sequences represented two new genotypes, called <i>Cryptosporidium</i> bat genotypes XIX and XX, were identified.</p><h3>Conclusions</h3><p>This study describes the detection of two novel <i>Cryptosporidium</i> bat genotypes, in two species of bats from a region of Colombia, requiring further studies to determine the relationhip between <i>Cryptosporidium</i> and bats in Colombia.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"68 3\",\"pages\":\"676 - 682\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s11686-023-00697-8.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-023-00697-8\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-023-00697-8","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Molecular Detection and Genotyping of Cryptosporidium spp. Isolates from Bats in Colombia
Purpose
Cryptosporidiosis is a zoonotic infectious disease caused by the protozoan parasite Cryptosporidium spp., frequently found in several animal species, including bats. Several Cryptosporidium genotypes have been described in bats worldwide, suggesting that bats are infected by host-specific Cryptosporidium spp. To date, there are no published reports about Cryptosporidium spp. in bats from Colombia. Therefore, this study aimed to determine the presence and molecular diversity of Cryptosporidium spp. in Colombian bats.
Methods
A total of 63 gut samples from three bat species served for molecular detection of Cryptosporidium spp. 18S rDNA gene by qPCR. The sequenced amplicons were used in subsequent phylogenetic analyses to identify them as species or genotypes.
Results
Cryptosporidium spp. qPCR detection occurred in 9.5% (6/63) of bat intestines, and four sequences represented two new genotypes, called Cryptosporidium bat genotypes XIX and XX, were identified.
Conclusions
This study describes the detection of two novel Cryptosporidium bat genotypes, in two species of bats from a region of Colombia, requiring further studies to determine the relationhip between Cryptosporidium and bats in Colombia.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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