基于挂锁探针的靶向原位测序:方法和应用综述。

IF 7.7 2区 生物学 Q1 GENETICS & HEREDITY
Anastasia Magoulopoulou, Sergio Marco Salas, Katarína Tiklová, Erik Reinhold Samuelsson, Markus M Hilscher, Mats Nilsson
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引用次数: 1

摘要

阐明基因表达的时空变化一直是健康、发育和疾病研究的重要目标。在新兴的空间分辨转录组学领域,基因表达谱是在组织结构维持的情况下获得的,有时是在细胞分辨率上。这使得空间细胞图谱的发展、细胞间相互作用的研究和原位细胞分型成为可能。在这篇综述中,我们重点介绍了基于挂锁探针的原位测序,这是一种靶向空间分解的转录组学方法。我们总结了最近的方法和计算工具的发展,并讨论了关键的应用。我们还讨论了与其他方法的兼容性以及与未来应用的多组平台的集成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Padlock Probe-Based Targeted In Situ Sequencing: Overview of Methods and Applications.

Elucidating spatiotemporal changes in gene expression has been an essential goal in studies of health, development, and disease. In the emerging field of spatially resolved transcriptomics, gene expression profiles are acquired with the tissue architecture maintained, sometimes at cellular resolution. This has allowed for the development of spatial cell atlases, studies of cell-cell interactions, and in situ cell typing. In this review, we focus on padlock probe-based in situ sequencing, which is a targeted spatially resolved transcriptomic method. We summarize recent methodological and computational tool developments and discuss key applications. We also discuss compatibility with other methods and integration with multiomic platforms for future applications.

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来源期刊
CiteScore
14.90
自引率
1.10%
发文量
29
期刊介绍: Since its inception in 2000, the Annual Review of Genomics and Human Genetics has been dedicated to showcasing significant developments in genomics as they pertain to human genetics and the human genome. The journal emphasizes genomic technology, genome structure and function, genetic modification, human variation and population genetics, human evolution, and various aspects of human genetic diseases, including individualized medicine.
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