{"title":"肌酸化学交换饱和转移(Cr-CEST)成像可评估顺铂致睾丸损伤。","authors":"Sohei Kuribayashi, Shigeyoshi Saito, Reika Sawaya, Yusuke Takahashi, Hidetaka Kioka, Kentaro Takezawa, Hiroshi Kiuchi, Shinichiro Fukuhara, Norio Nonomura","doi":"10.2463/mrms.mp.2021-0125","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to investigate the ability of creatine-chemical exchange saturation transfer (Cr-CEST) technique assessed through 7-T MRI to evaluate cisplatin-induced testicular damage.</p><p><strong>Methods: </strong>We used 8-10 weeks C57BL/6 mice (n = 10) that were divided into a control group (n = 5) and a cisplatin-treated group (n = 5). The cisplatin group received cisplatin at a dose of 15 mg/kg, via intraperitoneal injection, while the control group received saline. MR images of mouse testes were acquired under anesthesia 18 days after the injection using a horizontal 7-T scanner. The pulse sequence consisted of rapid acquisition with a relaxation enhancement (RARE) with magnetization transfer. The Z-spectra were collected using a 2000-ms saturation pulse at a B<sub>1</sub> amplitude of 1.2 μT, with frequencies varying from -4.8 to +4.8 parts per million (ppm). Maps of magnetization transfer ratio with asymmetric analysis (MTR<sub>asym</sub>) were reconstructed at a Cr metabolite concentration of 1.8 ppm.</p><p><strong>Results: </strong>The Cr-CEST effect was significantly reduced in the cisplatin-treated group compared to the control group (MTR<sub>asym</sub> of control mice vs. cisplatin-treated mice: 6.9 [6-7.5] vs. 5.2 [4-5.5], P = 0.008). Correlation analysis revealed a strong correlation between the Cr-CEST effect and the pathological score (ρ = 0.93, P < 0.001).</p><p><strong>Conclusion: </strong>Cr-CEST MRI can be useful for the evaluation of cisplatin-induced testicular damage in mice.</p>","PeriodicalId":18119,"journal":{"name":"Magnetic Resonance in Medical Sciences","volume":"22 3","pages":"345-351"},"PeriodicalIF":2.5000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/98/mrms-22-345.PMC10449556.pdf","citationCount":"3","resultStr":"{\"title\":\"Creatine Chemical Exchange Saturation Transfer (Cr-CEST) Imaging Can Evaluate Cisplatin-induced Testicular Damage.\",\"authors\":\"Sohei Kuribayashi, Shigeyoshi Saito, Reika Sawaya, Yusuke Takahashi, Hidetaka Kioka, Kentaro Takezawa, Hiroshi Kiuchi, Shinichiro Fukuhara, Norio Nonomura\",\"doi\":\"10.2463/mrms.mp.2021-0125\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>This study aimed to investigate the ability of creatine-chemical exchange saturation transfer (Cr-CEST) technique assessed through 7-T MRI to evaluate cisplatin-induced testicular damage.</p><p><strong>Methods: </strong>We used 8-10 weeks C57BL/6 mice (n = 10) that were divided into a control group (n = 5) and a cisplatin-treated group (n = 5). The cisplatin group received cisplatin at a dose of 15 mg/kg, via intraperitoneal injection, while the control group received saline. MR images of mouse testes were acquired under anesthesia 18 days after the injection using a horizontal 7-T scanner. The pulse sequence consisted of rapid acquisition with a relaxation enhancement (RARE) with magnetization transfer. The Z-spectra were collected using a 2000-ms saturation pulse at a B<sub>1</sub> amplitude of 1.2 μT, with frequencies varying from -4.8 to +4.8 parts per million (ppm). Maps of magnetization transfer ratio with asymmetric analysis (MTR<sub>asym</sub>) were reconstructed at a Cr metabolite concentration of 1.8 ppm.</p><p><strong>Results: </strong>The Cr-CEST effect was significantly reduced in the cisplatin-treated group compared to the control group (MTR<sub>asym</sub> of control mice vs. cisplatin-treated mice: 6.9 [6-7.5] vs. 5.2 [4-5.5], P = 0.008). Correlation analysis revealed a strong correlation between the Cr-CEST effect and the pathological score (ρ = 0.93, P < 0.001).</p><p><strong>Conclusion: </strong>Cr-CEST MRI can be useful for the evaluation of cisplatin-induced testicular damage in mice.</p>\",\"PeriodicalId\":18119,\"journal\":{\"name\":\"Magnetic Resonance in Medical Sciences\",\"volume\":\"22 3\",\"pages\":\"345-351\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/98/mrms-22-345.PMC10449556.pdf\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Magnetic Resonance in Medical Sciences\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2463/mrms.mp.2021-0125\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Magnetic Resonance in Medical Sciences","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2463/mrms.mp.2021-0125","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}
Creatine Chemical Exchange Saturation Transfer (Cr-CEST) Imaging Can Evaluate Cisplatin-induced Testicular Damage.
Purpose: This study aimed to investigate the ability of creatine-chemical exchange saturation transfer (Cr-CEST) technique assessed through 7-T MRI to evaluate cisplatin-induced testicular damage.
Methods: We used 8-10 weeks C57BL/6 mice (n = 10) that were divided into a control group (n = 5) and a cisplatin-treated group (n = 5). The cisplatin group received cisplatin at a dose of 15 mg/kg, via intraperitoneal injection, while the control group received saline. MR images of mouse testes were acquired under anesthesia 18 days after the injection using a horizontal 7-T scanner. The pulse sequence consisted of rapid acquisition with a relaxation enhancement (RARE) with magnetization transfer. The Z-spectra were collected using a 2000-ms saturation pulse at a B1 amplitude of 1.2 μT, with frequencies varying from -4.8 to +4.8 parts per million (ppm). Maps of magnetization transfer ratio with asymmetric analysis (MTRasym) were reconstructed at a Cr metabolite concentration of 1.8 ppm.
Results: The Cr-CEST effect was significantly reduced in the cisplatin-treated group compared to the control group (MTRasym of control mice vs. cisplatin-treated mice: 6.9 [6-7.5] vs. 5.2 [4-5.5], P = 0.008). Correlation analysis revealed a strong correlation between the Cr-CEST effect and the pathological score (ρ = 0.93, P < 0.001).
Conclusion: Cr-CEST MRI can be useful for the evaluation of cisplatin-induced testicular damage in mice.
期刊介绍：
Magnetic Resonance in Medical Sciences (MRMS or Magn
Reson Med Sci) is an international journal pursuing the
publication of original articles contributing to the progress
of magnetic resonance in the field of biomedical sciences
including technical developments and clinical applications.
MRMS is an official journal of the Japanese Society for
Magnetic Resonance in Medicine (JSMRM).
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